Ligase detection reaction

Recent work by Lowe et al. used the ligase detection reaction (LDR) to detect SNPs in a multiplexed manner using SERS [59]. The so called LDR-SERS approach involved the functionalization of an oligonucleotide primer (which binds downstream of the SNP) with an amine to allow it to attach to the surface of nanoparticles after the ligation. A second oligonucleotide primer sequence... 

Lowe AJ, Huh YS, Strckland AD, Erickson D, Ban CA (2010) Multiplex single nucleotide polymorphism genotyping utilizing ligase detection reaction coupled surface enhanced Raman spectroscopy. Anal Chem 82(13) 5810-5814... 

Huh, Y.S., Lowe, A.J., Strickland, A.D., Batt, C.A., and Erickson, D. (2009) Surface-enhanced Raman scattering based ligase detection reaction. Journal ofthe American Chemical Society, 131, 2208-2213. 

Y. S. Huh, A. J. Lowe, A. D. Striekland, C. A. Batt, and D. Erickson, Surface-Enhanced Raman Scattering Based Ligase Detection Reaction. Journal of the American Chemical Society 131 p. 2208-2213 (2009). 

Thomas, G., et al., Capillary and microelectrophoretic separations of ligase detection reaction products produced from low-abundant point mutations in genomic DNA, Electrophoresis, 25,1668, 2004. BraziU, S.A. and Kuhr, W.G., A single base extension technique for the analysis of known mutations utilizing capillary gel electrophoresis with electrochemical detection. Anal Chem, 74, 3421, 2002. 

Bust , E., Bordoni, R., Castigiioni, B., Monciardini, P., Sosio, M., Donadio, S., Consoiandi, C., Rossi Bernard , L., Battaglia, C., De Beilis, G. (2002) Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction). BMC Microbiol, 2, 27. 

Both target and signal amplification systems have been successfully employed to detect and quantitate specific nucleic acid sequences in clinical specimens. Polymerase chain reaction (PCR), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), strand displacement amplification (SDA), and ligase chain reaction (LCR) are all examples of enzyme-mediated, target amplification strategies that are capable of producing billions of... 

Mycobacteria of the Mycobacterium avium complex are implicated in disseminated bacterial infections in AIDS patients. RFLP studies followed by hybridization with radiolabeled probe specific for an insertion sequence in M. avium (IS 1311) have been useful for typing M. avium stains (R2). A variety of molecular techniques are available for the diagnosis of Chlamydia trachomatis infection. In addition to PCR, a method based on the ligase chain reaction has also been found to be sensitive to the detection of C. trachomatis infection in urine specimens collected from male and female subjects (VI). The differentiation between low-risk genotypes of human papilloma virus (HPV 6 or 11) from genotypes of high... 

HCV and HIV-1). The bDNA assay is being much employed for the quantification of messenger RNA. Moreover, for the detection of viral and pathogenic disorders based on alkahne-phosphatase-sensitive dioxetanes, several assay methods are available these include the Polymerase-Chain-Reaction (PCR) amphfication, probe ligation, strand-displacement amplification and the ligase chain reaction. ... 

Cheng et al. [211] described an NCE-LIF system for the rapid separation and quantitative detection of ligase chain reaction (LCR) products amplified from the lac I gene on a silicon-glass chip. LCR is a useful molecular technique for... 

There are other techniques that can be used to amplify DNA. For instance, the ligase chain reaction (LCR) was achieved on a Si-glass chip for detecting known... 

Buimer M, van Doornum GJ, Ching S, Peerbooms PG, Plier PK, Ram D, et al. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by ligase chain reaction-based assays with clinical specimens from various sites implications for diagnostic testing and screening, J Clin Microbiol 1996 34 2395-400. 

Puolakkainen M, Hiltunen-Back E, Reunala T, Suhonen S, Lahteenmaki P, Lehtinen M, et al. Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction test, in detection of urogenital Chlamydia trachomatis infection. J Clin Microbiol 1998 36 1489-93. 

Lou, X. J., Ranaro, N. J., Wilding, R., Fortina, R, and Kricka, L. J., Mutation detection using ligase chain reaction in passivated sdicon-glass microchips and microchip capillary electrophoresis. Biotechniques, 37, 392, 2004. 

Ch. 35. Nonradioactive Oligonucleotide Probes for Detecting Products of the Ligase Chain Reaction,... 

The ligase chain reaction (LCR) is a novel DNA amplification system developed jointly by Abbott Laboratories (North Chicago, EL) and Biotechnica International, Inc. (Kansas City, MO) (i). By amplification with specially haptenated detection probes, LCR can detect less than 100 target DNA molecules in a nonradioactive assay format. 

Earlier reports on the use of MALDI-TOF-MS for the detection of mutahons and/or sequence changes have employed more conventional techniques such as restriction endonuclease digests [146] or the ligase chain reaction [147]. Inihally, allele-specific hybridization was also combined with mass spectrometric detection. Smith and coworkers, for example, used pephde NA probes hybridized against an immobilized PCR template for genotyping [148]. Finally, the direct measurement of PCR products has been attempted as a means of NA analysis and genotyping [17, 24, 92, 149, 150]. 

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