Lectins sources

Lectin Source Sugar specificity Eluting sugar... 

Vomholt W, Hartmann M, Keusgen M (2007) SPR studies of carbohydrate-lectin interactions as useful tool for screening on lectin sources. Biosens Bioelectron 22 2983-2988... 

Chemical class Lectin Source Abrus precatorius. Molecular formula C12H14N2 O2. Molecular weight 216.28. Melting point 216.28 deg C. 

Nr. Lectin Source Taxonomic Name Common Name Carbohydrate Specificity Positive Reaction in Non- Reduced Form Positive Reaction in Reduced Form Reference/ Figure... 

LiENERi E, DONATUCCi DA, TARCZA J c (1984) Starch blockers apotential source oftrypsin inhibitors and lectins. Am J Clin Nutr. 39 196-200. 

Crude protein sources containing one glycoprotein usually contain multiple glycoproteins. In most such instances, lectin-based affinity systems will result in the co-purification of several such glycoproteins. 

GM-CSF is undetectable in the serum of normal humans, and no normal cells have been shown to express this protein constitutively. Some transformed cells may constitutively express GM-CSF, and it is actively synthesised and secreted by antigen- and lectin-stimulated T cells and by endothelial cells and fibroblasts exposed to TNF, IL-1 or endotoxin. Other sources of GM-CSF include stimulated B lymphocytes, macrophages, mast cells and osteoblasts, whilst TNF and IL-1 can stimulate its production by acute myeloid leukaemia cells. Some solid tumours and synovial cells from rheumatoid joints may also express GM-CSF and this may be important in disease pathology. 

The software required for the quantitative evaluation and wavelength normalisation of Laue data has been successfully developed at Daresbury since the feasibility of recording full white beam Laue patterns from protein crystals was established. The software and the method have been tested using Laue data from crystals of pea lectin. As an example of the statistical quality (to 3 A resolution) the mean fractional change on F between monochromatic and Laue pea lectin data was 11 % and the same quantity between conventional source monochromatic and SR monochromatic pea lectin data is 8% (Helliwell et al., 1986). 

Specific detection of oligosaccharides is possible using lectins, carbohydrate-binding proteins from very different sources. A se-... 

Source Weiss, W.l. Drickamer, K. (1996) Structural basis of lectin-carbohydrate recognition, Annu. Rev. Biochem. 65, 441-473,... 

Blood Groups XLIII. The Interaction of Blood Group Substances from Various Sources with a Plant Lectin, Concanavalin A, J. Immunol. (1969) 102,1354. 

One hemagglutination inhibition unit (HAIU) is defined as the minimum amount of saccharide necessary to inhibit completely three hemagglutination units of lectin. The purest available sugars were obtained from various sources 2-acetamido-2-deoxy-D-glucose (GlcNAc), GalNAc, and 2-acetamido-2-deoxy-g-mannose (ManNAc) from Sigma Chemical Co., St. Louis, Missouri, and D-Gal and lactose from Fisher Scientific Co., Fair Lawn, New Jersey. 

Lectin name Source Sugar specificity Interacting antibody... 

Although seeds are the common source of lectin activity, there are reports (some contradictory) of agglutinins occurring in leaves, stems, and roots.77,94 For example, black-locust (Robinia pseudoaccacia) lectin160 was isolated from its bark, potato (Solanum tuberosum) lectin161 from its tubers, and poke-weed (Phytolacca americanum) mitogen162 from its roots. A new class of plant cell-wall and membrane-bound lectins ( /3-lectins ) has been described.116,163,164 A treatise collected several schemes for the purification of lectins.165,1658... 

Balding and Gold599 studied the agglutination of various, red bloodcell types by extracts of Sophora japonica seeds from varied geographical sources. Whereas a purified lectin sample from R. D. Poretz (seeds from a supplier in the United States) and lectin from Japanese seeds did not agglutinate O cells, a sample from Portugal did. All three lectin... 

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