Laser desorption/ionization mass spectrometry sample preparation

Kussmann M, Nordhoff E, Rahbek-Nielsen H, Haebel S, Rossel-Larsen M, Jakobsen L, Gobom J, Mirgorodskaya E, Kroll-Kristensen A, Palm L, Roepstorff P (1997) Matrix-assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptides and protein analytes. J Mass Spectrom 32 593-601... [Pg.417]

Mirgorodskaya, E., Braeuer, C., Fucini, P, Lehrach, FI. and Gobom, J. (2005a) Nanoflow liquid chromatography coupled to matrix-assisted laser desorption/ionization mass spectrometry Sample preparation, data anlysis, and application to the analysis of complex peptide mixtures. Proteomics 5, 399-408. [Pg.378]

Wetzel, S.J., Guttman, C.M. and Flynn, K.M., The influence of electrospray deposition in matrix-assisted laser desorption/ionization mass spectrometry sample preparation for synthetic polymers. Rapid Commun Mass Spectrom, 18 (2004) 1139-1146. [Pg.566]

Kussmann, M., Nordhoff, E., Rahbek-Nielsen, H., Haebel, S., Rossel-Larsen, M., Jakobsen, L., Gobom, J., Mirgorodskaya, E., Kroll-Kristensen, A., Pahn, L., and Roepstorff, R, Matrix-assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptide and protein analytes, Journal of Mass Spectrometry, 32, 593-601, 1997. [Pg.1368]

Vaidyanathan, S. Winder, C. L. Wade, S. C. Kell, D. B. Goodacre, R. Sample preparation in matrix-assisted laser desorption/ionization mass spectrometry of whole bacterial cells and the detection of high mass (>20kDa) proteins. Rapid Comm. Mass Spectrom. 2002,16,1276-1286. [Pg.150]

Schwartz S, Reyzer M, Caprioli R. Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry practical aspects of sample preparation. /. Mass. Spectrom. 2003 38 699-708. [Pg.388]

Sample Preparation Using Synthetic Membranes for the Study of Biopolymers By Matrix Assisted Laser Desorption/Ionization Mass Spectrometry... [Pg.143]

Also the use of an EWOD system for the automated sample preparation of peptides and proteins for matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) was reported. In that work, standard MALDI-MS reagents, analytes, concentrations, and recipes have been demonstrated to be compatible with the EWOD technology, and mass spectra comparable to those collected by conventional methods were obtained [271]. Also a PCR assay has been realized on the platform by temperature cycling of a droplet at rest [272]. Additional informations about the EWOD platform can be found in a comprehensive review [273]. [Pg.347]

Fukuzawa, S., Asanuma, M., Tachibana, K. and Flirota, FI., On-probe sample preparation without washes for matrix-assisted laser desorption/ionization mass spectrometry using an anion exchange medium. Anal Chem, 77 (2005) 5750-5754. [Pg.562]

Haddleton, D. M., Waterson, C., and Derrick, P. Comment A Simple, Low-cost, Air-spray Method for Improved Sample Preparation for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry of Derivatised Poly(ethylene glycol), Eur. Mass. Specirom., 4, 203,1998. [Pg.513]

Gobom,)., Nordhoff, E., Mirgorodskaya, E., Ekman, R., and Roepstorff, P. (1999) Sample purification and preparation technique based on nano-scale reversed-phase columns for the sensitive analysis of complex peptide mixtures by matrix-assisted laser desorption/ ionization mass spectrometry. J. Mass Spectrom., 34,105-116. [Pg.126]

Matrix-assisted laser desorption-ionization mass spectrometry (MALDI-MS) MALDI-MS technique enables ionization a broad range of materials, with high sensitivity, broad mass range of materials, fast sample preparation, and the absence of fragmentation. [Pg.357]

Experimental considerations Sample preparation and data evaluation are similar to membrane osmometry. Since there is no lower cut-off as in membrane osmometry, the method is very sensitive to low molar mass impurities like residual solvent and monomers. As a consequence, the method is more suitable for oligomers and short polymers with molar masses up to (M)n 50kg/mol. Today, vapour pressure osmometry faces strong competition from mass spectrometry techniques such as matrix-assisted laser desorption ionisation mass spectrometry (MALDI-MS) [20,21]. Nevertheless, vapour pressure osmometry still has advantages in cases where fragmentation issues or molar mass-dependent desorption and ionization probabilities come into play. [Pg.217]