Laser desorption/ionization mass spectrometry layers

A toluene solution of polymer 15 was added to an aqueous mixture of peptides. After effective equUibration, the heterogeneous mixture was allowed to separate and the organic layer was analyzed by matrix assisted laser desorption ionization mass spectrometry analysis. Because the pH of the aqueous solution was 7.1, the peptides with pis above 7.1 were extracted and the peptides with pis below 7.1... 

Matrix Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS) Mass spectra of native and denatured antibodies were obtained with a PerSeptive Biosystems (Farmingham, MA.) Voyager Elite mass spectrometer operated in the linear mode with a Laser Sciences Inc., 337 nm nitrogen laser. hAB-1 was denatured by boiling the sample in 1.0 M guanidine-HCl, 50 mM Tris pH 7.5 buffer. Native and denatured samples were diluted with 20 mM Tris, 10 mM octylglucoside (Tris/OG) pH 6.8 buffer prior to MALDl-MS analysis. Proteins were spotted on the sample plate as a sandwich between two layers of the matrix. The bottom layer consisted of 100 mM sinapinic acid in acetonitrile and the top layer consisted of 50 mM sinapinic acid in 30% acetonitrile / 70% H2O / 0.07% TFA. The m/z scale of the instrument was calibrated using a Hewlett-Packard protein standard mixture. 

Guser, A. J, A. Proctor, Y. J. Rabinovich, D. M. Hercules Thin-Layer Chromatography Combined with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry , Analytical Chemistry, Vol. 67, No. 11, June 1,1805-1814 (1995)... 

Stults, J. (1995). Matrix-assisted Laser-desorption Ionization Mass Spectrometry (MALDI-MS), Current Opinion in Structural Biology 5 691-698. The review contains a host of references for special topics, such as differentiation of sulfate and phosphate groups, MALDI with infrared lasers and succinic acid matrix, subfem-tomolar detection thresholds with a thin layer matrix, highly sensitive protein detection in the attomolar range, and high-resolution mass spectrometry. 

Nicola AJ, Gusev AI, Hercules DM. Direct quantitative analysis from thin-layer chromatography plates using matrix-assisted laser desorption/ionization mass spectrometry. Appl Spectrosc 1996 50 1479-1482. 

Thin-layer chromatography combined with matrix- assisted laser desorption ionization mass spectrometry. Anal. 

Weyermann, C. Marquis, R. Mazzella, M. Spengler, B. Differentiation of blue ballpoint pen inks by laser desorption ionization mass spectrometry and high performance thin layer chromatography. J. Forens. Sci. 2007, 52, 216-220. 

Chen, C.-C., Yang, Y.-L., Ou, C.-L., Chou, C.-H., Liaw, C.-C., Lin, P.-C. (2013) Direct Monitoring of Chemical Transformations by Combining Thin Layer Chromatography with Nanoparticle-assisted Laser Desorption/Ionization Mass Spectrometry. Analyst 138 1379-1385. 

Salo, P.K., Salomies, H., Harju, K., Ketola, R.A., Kotiaho, T., Yli-Kauhaluoma, I, Kostiainen, R. (2005) Analysis of small molecules by ultra thin-layer chromatography-atmospheric pressure matrix-assisted laser desorption/ ionization mass spectrometry. Journal of the American Society for Mass Spectrometry,16, 906-915. 

Lin, S., Huang, M., Chang, H., Shiea, J. (2007) Using electrospray-assisted laser desorption/ionization mass spectrometry to characterize organic compounds separated on thin-layer chromatography plates. Analytical Chemistry, 79, 8789-8795. 

Hayen, H., Vohner, D.A. (2005) Rapid identification of siderophores by combined thin-layer chromatography/ matrix-assisted laser desorption/ionization mass spectrometry. Rapid Communications in Mass Spectrometry RCM, 19,111-120. 

Kuwayama, K., Tsujikawa, K., Miyaguchi, H., Kanamori, T., Iwata, Y.T., and Inoue, H. 2012, Rapid, simple, and highly sensitive analysis of drugs in biological samples using thin-layer chromatography coupled with matrix-assisted laser desorption/ionization mass spectrometry. Ana/. Bioanal. Chem., 402 1257-1267. 

Gusev, A.I., Proctor, A., Rabinovich, Y.I., and Hercules, D.M. 1995. Thin-layer chromatography combined with matrix-assisted laser desorption/ionization mass spectrometry. Anal. Chem., 67 1805-1814. 

High-Throughput Thin-Layer Chromatography-Electrospray Laser Desorption Ionization/Mass Spectrometry Analysis... 

Shariatgorji, M., Spacil, Z., Maddalo, G., Cardenas, L.B., and Hag, L.L. 2009. Matrix-free thin-layer chromatography-laser desorption ionization mass spectrometry for facile separation and identification of medicinal alkaloids, Rapid Commun. Mass Spectrom., 23 3655-3660. 

Zhang, J., Zhou, Z., Yang, J., Zhangm, W., Bai, Y, and Liu, H. 2012. Thin layer chromatography-plasma assisted multiwavelength laser desorption ionization mass spectrometry for facile separation and selective identification of low molecular weight compounds. Ana/. Chem., 84 1496—1503. 

APPLICATIONS OF THIN-LAYER CHROMATOGRAPHY COMBINED WITH MATRIX-ASSISTED LASER DESORPTION IONIZATION-MASS SPECTROMETRY... 

Qureshi, M.N., Stecher, G., Huck, C., and Bonn, G.K. 2010. Online coupling of thin layer chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Synthesis and application of a new material for the identification of carbohydrates by thin layer chromatography/matrix free material enhanced laser desorption/ ionization mass spectrometry. Rapid Commun. Mass Spectrom., 24 2759-2764. 

Piret G, Kim D, Drobecq H, Coffinier Y, Melnyk O, Schmuki P, Boukherroub R (2012) Surface-assisted laser desorption/ionization mass spectrometry on titanium dioxide (Ti02) nanotube layers. Analyst 137 3058-3063... 

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