Kirby-Bauer antimicrobial

Figure 4. Kirby-Bauer Antimicrobial Susceptibility Test, Klebsiella pneumoniae, showing inhibition of bacterial growth around the test article...

The Kirby-Bauer technique (Black 2002) was used to test for antimicrobial activity... 

It is important to point out that although the caprylic acid/oleic acid emulsion demonstrated small, but measurable, antimicrobial activity in the liquid phase (Baker Suspension Test), and the Kirby-Bauer Plate test showed marginal antimicrobial... 

Antimicrobial testing was performed using the Kirby-Bauer Disk assay as described by Hendrix (1) and results summarized in Table 3. 

The agar diffusion method (Kirby—Bauer) is also sometimes used for the evaluation of antibacterial activity of textiles. This is a relatively quick and easily executed semiquantitative method to determine antibacterial activity of diffusible antimicrobial agents on treated textile material. The bacteria are grown in nutrient broth medium and after appropriate dilution (e.g., lOOx) from the culture, test organisms are swabbed over the surface of agar plates. Ten-millimetre-diameter disks of the test fabric and control fabric are then gently pressed onto the surface of the plate. The plates are then incubated at 37 °C for 18—24 h. The antibacterial activity of the fabrics is demonstrated by the diameter of the zone of inhibition in comparison to the control textile sample. 

NOTE 1 SECTION 35 is to be used to record disc sensitivities for clinical applications (eg., the methods of Bauer et. al., 1966, commonly referred to as Kirby-Bauer or Bauer-Kirby Methods). Other tests of antimicrobial sensitivity or resistance should be coded under SECTION 19/40.]... 

The ZOI test, also widely known as the Kirby-Bauer disk diffusion test, is a fast in vitro but semiquantitative test [169], The original purpose of this test was to replace the MIC test for small molecule antibiotic efficacy [169], Soon, this method was adopted and modified to evaluate antimicrobial efficacy of silver and polymeric devices with eluting antimicrobial agents [170], Conunonly used eluting antimicrobial agents are zinc salt/particles [171-173], silver salt/particles [173-177], and chlorhexi-dine [178,179], These antimicrobial agents can be compounded/blended into polyurethanes or coated/adsorbed on polyurethanes. 

Boyle VJ, Fancher ME, Ross RW. Rapid, modified Kirby-Bauer susceptibUity test with single, high-concentration antimicrobial disks. Antimicrob Agents Chemother 1973 3 418-24. 

The library of silver-NHC compounds has been greatly expanded due to the contributions of Tacke and coworkers (13a-21) [13-17] and Roland et al. (22a-25b) [18]. Compounds 13a-21 (Figure 6.1), all bearing the acetate ligand, were evaluated for their antimicrobial efficacy against S. aureus and . coli using a qualitative Kirby-Bauer disk-diffusion method. The imidazolium salt precursors, silver acetate, and the vehicle (dimethylsulfoxide) served as controls. The results of the tests were mixed, with a number of compounds having a weak... 

Bauer, A. W., M. M. Kirby, J.C. Sherris and M. Turck. 1966. Antibiotic susceptibility and testing by a standardized single disc method. Am. J. Clin. Pathol. 45 493-496.) New feature numbers have been added to reflect the changes and additions as published by the National Committee for Clinical Laboratory Standards (NCCLS). (See Performance Standards for Antimicrobial Disc Susceptibility Tests. Second edition May, 1981 NCCLS, Vol. 1 No. 6 771 E. Lancaster Avenue Villanova, PA 19805.) (See also Thornsberry, C. 1980. Disc agar diffusion susceptibility test. 

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