Jurkat cell surfaces

The use of this reaction in the biological context was first demonstrated for the chemospecific labeling of Jurkat cell surfaces [63]. Metabolic engineering with N-acetylmannosamine derivative 40c was used to incorporate azides into sialic acid groups on cell surfaces. The cells were then incubated with biotinylated phosphine 49, and the extent of the reaction was quantified by flow cytometry after treatment with fluorescent avidin. Importantly, neither the azide nor the phosphine displayed any reactivity with the cell-surface groups in the absence of its reactive partner. In addition, the cells showed unchanged growth rates after modification. 

Jurkat cells have been lysed in a flow stream in a glass microchip for cell content analysis. After cell lysis, the two preloaded fluorescent dyes and their metabolites were released from the cells and separated by CE (see Figure 8.36). To prevent cell adhesion, the glass channel surface was modified by adsorbing Pluronic F-127 to the channels. In addition, to avoid blockage of adhered cell debris and to improve migration time stability, an emulsification agent, such as Pluronic P84, was added to the separation buffer [1176],... 

It could be shown that PBCA particles are also internalized by HeLa, Jurkat, and mesenchymal stem cells (MSCs) however, the cellular uptake kinetics are different for HeLa and Jurkat cells (see Fig. 6) [35, 36]. While the particle size has a significant impact on particle uptake in HeLa cells, Jurkat cells are more sensitive towards surface functionalization. Especially the methoxypolyfethylene glycol) (MePEG)-fimctionalized particles are internalized to a lesser extent than the rest of the investigated particles (non-functionalized, phenylalanine-functionalized). 

One way to modify the cell-surface molecules is by introducing nonnatural monosugars into the biosynthetic pathways that build up polysaccharides (Figure 46.3) [15,16]. By introducing monosaccharides with synthetic ketone groups, Bertozzi et al. were able to engineer previously nonadherent Jurkat cells to adhere to hpid bilayers decorated with azides [17]. These researchers have also demonstrated that a... 

The final test of the applicability of this technology for bioconjugation applications came when these workers introduced the corresponding JV-azidoacetyl sialic acid s (SiaNAz) residues to the cell-surface glycoproteins of Jurkat cells (a human T lymphoma cell line). The cells were then incubated with various concentrations of the biotinylated cyclooctene analog (Scheme 9.30b), and cells expressing the SiaNAz residues showed a marked dose-dependent increase in their fluorescence when treated with the biotinylated reagent. 

Lactotransferrin receptors. The existence of a lactotransferrin receptor was first demonstrated by Van Snick and Masson in 1976 [191] at the surface of mouse peritoneal macrophages and lymphocytes. Since this discovery, the presence of lactotransferrin receptors has been demonstrated at the surface of various cells (for reviews, see refs. [156,158,192,193]) rabbit [48], mouse [165,166], monkey [167] and human [168] enterocytes human HT29 and Caco-2 enterocyte cell lines [194] human monocytes (reviewed in ref. [195]), human alveolar macrophages [196], human neutrophils [195,197], human resting lymphocytes [197], human activated lymphocytes [189], human Jurkat T cell line [190], human epithelial mammary cell line [198], human platelets [199,200] and megakaryocytes [201], hepatocytes [202,203] and in bacteria (for a review see refs. [204,205]). 

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