Jurkat

ROY s, SEN c K, KOBUCHi H and PACKER L (1998) Antioxidant regnlation of phorbol ester-indnced adhesion of hiunan Jurkat T-cells to endothehal cells Free Radical Biology and Medicine 25, 229—41. 

SPINOZZI F, PAGLIACCI M C, MIGLIORATI G, MORACA R, GRIGANI F, RICCARDI C, NICOLETTI I (1994) The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. LeukRes. 18 431-9. 

Inayat-Hussain SH, Osman AB, Din LB, Ali AM, Snowden RT, MacFarlane M, Cain K. Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Lett 1999 456 379-383. 

Jurkat cells Aggregation-dependent cytotoxicity Contaminant-dependent cytotoxicity [163]... 

De Nicola, M. et al. (2008) Carbon nanotubes on Jurkat cells effects on cell viability and plasma membrane potential. Journal of Physics Condensed Matter,... 

Harnett, M.M., Deehan, M.R., Williams, D.M. and Harnett, W. (1998a) Induction of signalling anergy via the TCR in Jurkat T cells by pre-exposure to a filarial nematode secreted product. Parasite Immunology 20, 551-564. 

Journet A et al. Towards a human repertoire of monocytic lysosomal proteins. Electrophoresis 2000 21 3411-3419. Soskic V et al. Functional proteomics analysis of signal transduction pathways of the platelet-derived growth factor beta receptor. Biochemistry 1999 38 1757-1764. Thiede B et al. A two dimensional electrophoresis database of a human Jurkat T-cell line. Electrophoresis 2000 21 2713-2720. 

Table 14.1 IL-2 reporter gene assay (transfected Jurkat T cells) activity of pateamine A and derivatives... 

As was the case for most other cytokines, medical appraisal/use of IL-2 was initially impractical due to the minute quantities in which it is normally produced. Some transformed cell lines, most notably the Jurkat leukaemia cell line, produces IL-2 in increased quantities, and much of the IL-2 used for initial characterization studies was obtained from this source. Large-scale IL-2 production was made possible by recombinant DNA technologies. Although the IL-2 gene/cDNA has now been expressed in a wide variety of host systems, it was initially expressed in E. coli, and most products being clinically evaluated are obtained from that source. As mentioned previously, the absence of glycosylation on the recombinant product does not alter its biological activity. 

Lehmann-Horn, F. and Jurkat-Rott, K. Voltage-gated ion channels and hereditary disease. Physiol Rev. 79 1317-1372, 1999. 

Nakagawa, H. etal., Generation of hydrogen peroxide primarily contributes to the induction of Fe(II)-dependent apoptosis in Jurkat cells by (-)-epigallocatechin gallate, Carcinogenesis, 25, 1567, 2004. 

Espinoza, L.A. and Smulson, M.E., Macroarray analysis of the effects of JP-8 jet fuel on gene expression in Jurkat cells, Toxicol., 189, 181, 2003. 

Somlyo We did some work with David Trentham in which we looked for this in terms of modelling the lag phases, and at best we could come up with a Hill coefficient of 2. This was in smooth muscle, not Jurkat cells. My belief is that it is closer to 1. David wanted to be more conservative and said that it could be 2, but never 4 (Somlyo et al 1992). 

AS601245 has been reported to be an ATP-competitive inhibitor of JNK1, 2 and 3 with IC50 values = 150, 220 and 70 nM, respectively, with minimal to no activity in a panel of 25 other kinases [48]. In Jurkat T-cells, AS601245 at 10 pM inhibited IL-2 production induced by phorbol-12-myristate-l 3-acetate (PMA) by 90%. The weaker cell activity could be due to poor cell permeability. The oral bioavailability of AS601245 in rats was 38%. In mice, AS601245, when dosed at 60 mg/kg p.o. in a developed arthritis model induced by collagen, showed... 

The cytotoxic and photocytotoxic effects of two water-soluble fullerene derivatives, a dendritic CL mono-adduct and the malonic acid CL tris-adduct were tested on Jurkat cells when irradiated with UVA or UVB light (Rancan et al., 2002). The cell death was mainly caused by membrane damage and it was UV dose-dependent. Tris-malonic acid fullerene was found to be more phototoxic than the dendritic derivative. This result is in contrast to the singlet oxygen quantum yields determined for the two compounds. 

Rancan F, Rosan S, Boehm F, Cantrell A, Brellreich M, Schoenberger H, Hirsch A, Moussa F (2002) Cytotoxicity and photocytotoxicity of a dendritic C(60) mono-adduct and a malonic acid C(60) tris-adduct on Jurkat cells. J Photochem Photobiol B 67 157-162. 

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