Cell, adhesion prevention

Frixen, U. W., Behrens, J., Sachs, M., Eberle, G., Voss, B., Warda, A., Loech-ner, D. and Birchmeier, W. (1991). E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells. J. Cell Biol. 113, 173-185. 

U.H. Frixen, J. Behrens, M. Sachs, G. Eberle, B. Voss, A. Warda, D. Lochner and W. Birchmeier, E-Cadherin-mediated Cell-Cell Adhesion Prevents Invasiveness of Human Carcinoma Cells. J. Cell Biol, 113 (1991) 173-185. 

Hydroxyurea is a ribonucleotide reductase inhibitor that prevents DNA synthesis and traditionally has been used in chemotherapy regimens. Studies in the 1990s also found that hydroxyurea increases HbF levels as well as increasing the number of HbF-containing reticulocytes and intracellular HbF. Other beneficial effects of hydroxyurea include antioxidant properties, reduction of neutrophils and monocytes, increased intracellular water content leading to increased red cell deformability, decreased red cell adhesion to endothelium, and increased levels of nitric oxide, which is a regulator involved in physiologic disturbances.22... 

To suppress cell adhesion on a material surface, one approach is to inhibit the adsorption of proteins. SAMs of alkanethiols that carry oligo(ethylene glycol) (OEG) [69] and phosphorylcholine [46, 70, 71] have been shown to prevent... 

Some active materials are carriers for drugs (drug delivery systems), some have immobilized peptides to enable cell adhesion or migration, some are degradable by hydrolysis or by specific enzyme action. Some contain bioactive agents (e.g., heparin, thrombomodulin) to prevent coagulation or platelet activation while others incorporate bioactive groups to enhance osteo-conduction. Many include polyethylene oxide to retard protein adsorption and this is perhaps the closest we have come to a kind of inertness. 

ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM). Platelets are attracted to damaged endothelium where they adhere to prevent blood loss in a similar fashion to white blood cells, i.e. via adhesion molecule interactions, to form a clot (thrombus). 

Jurkat cells have been lysed in a flow stream in a glass microchip for cell content analysis. After cell lysis, the two preloaded fluorescent dyes and their metabolites were released from the cells and separated by CE (see Figure 8.36). To prevent cell adhesion, the glass channel surface was modified by adsorbing Pluronic F-127 to the channels. In addition, to avoid blockage of adhered cell debris and to improve migration time stability, an emulsification agent, such as Pluronic P84, was added to the separation buffer [1176],... 

In one report, Pluronics surfactant (tri-block copolymer of PEO-PPO-PEO) was used to prevent cell adhesion in a chip. This is because the center PPO block is hydrophobic and thus shields any hydrophobic surface on the chip. In addition, the end PEO groups are neutral but very hydrophilic, and will not interact with proteins and cells [278]. 

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