Proteomics functional analysis

Key Words Protein array biotinylation proteomics functional analysis surface capture p53 biotin carboxyl carrier protein fusion protein microarray DNA binding. 

Abdolzade-Bavil A, Hayes S, Goretzki L, Kroger M, Anders J, Hendriks R (2004) Convenient and versatile subcellular extraction procedure, that facilitates expression profiling and functional protein analysis. Proteomics 4 1397-1405 Bradford M (1976) A rapid and sensitive method forthe quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Analytical Biochem 248-254... 

Morandell, S., Stasyk, T., Grosstessner-Hain, K., Roitinger, E., Mechtler, K., Bonn, G. K., and Huber, L. A. Phosphoproteomics strategies for the functional analysis of signal transduction. Proteomics 6, 4047 1056, 2006. 

The goal of this chapter is to detail the need for such activity-based methods, and to describe the development and application of ABPP by highlighting several studies that have established the utility of this chemical proteomic method as a powerful strategy for the discovery and functional analysis of complex biological proteomes, as well as their individual constituents. 

The expression of a target gene in normal tissue from various vital organs can also be assessed using TMA panels. It is well known that mRNA levels do not reflect protein quantities and function, so proteomic analysis may help in making better decisions on targets to be developed for therapeutics [144],... 

Proteomics can be broadly defined as the identification, localization, and functional analysis of the protein make-up of cells. One of the major challenges faced, however, is lengthy sample preparation. Several methods have been developed to address this issue including the use of immobilized enzymes and acid-labile surfactants. Microwave heating can also play a valuable role. 

Flores FJ, Martin JF (2004) Iron-regulatory proteins DmdRl and DmdR2 of Streptomyces coelicolor form two different DNA-protein complexes with iron boxes. Biochem J 380 497-503 Flores FJ, Barreiro C, Coque JJR, Martin JF (2005) Functional analysis of Two divalent metal-dependent genes dmdRl and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants. FEBS J 272 725-735... 

Kato C, Kurihara T, Kobashi N, Yamane H, Nishiyama M (2004) Conversion of feedback regulation in aspartate kinase by domain exchange. Biochem Bioph Res Co 316 802-808 Kim YH, Park JS, Cho JY, Cho KM, Park YH, Lee J (2004) Proteomic response analysis of a threonine-overproducing mutant of Escherichia coli. Biochem J 381 823-829 Klaffl S, Eikmanns BJ (2010) Genetic and functional analysis of the soluble oxaloacetate decarboxylase from Corynebacterium glutamicum. J Bacterid 192 2604-2612 Komatsubara S, Kisumi M, Murata K, Chibata 1 (1978) Threonine production by regulatory mutants of Serratia marcescens. Appl Environ Microbiol 35 834-840 Kotaka M, Ren J, Lockyer M, Hawkins AR, Stammers DK (2006) Structures of R- and T-state Escherichia coli aspartokinase 111 mechanisms of the allosteric transition and inhibition by lysine. J Biol Chem 281 31544-31552... 

---