Jurkat leukemia

Cytotoxic activities of compoimd XVIII against human Jurkat leukemia... 

Cytotoxic activities of compound XXII against Human Jurkat leukemia (JLc) cells ... 

Tx is the sum of the hydrophobicity of X-substituents. A small hydrophobic effect of the X-substituents was observed against human Jurkat leukemia (JLc) cells but not against murine Lewis lung carcinoma (LL) cells. CMR is the most important determinant for the cytotoxic activities of these compounds (XXII) against both cell hnes and cannot be replace by Clog P. 

This hypothesis is consistent with the results of the aforecited study [78], which established a cytotoxic effect of DNIC with thiosulfate on human Jurkat leukemia... 

In parallel, Raubenheimer and coworkers developed new gold(I)-ferrocenyl complexes [11]. A series of cancer cell lines was tested with these, which includes HeLa cervix epithelioid carcinoma, CoLo 320 DM, Jurkat leukemia, and MCF-7 (breast cancer). The Jurkat cell lines were found to be the most responsive, while low sensitivity was observed for CoLo 320 DM. IC50 for HeLa and Jurkat were significant for complex 27 compared to cisplatin (Figure 7.10). 

SPINOZZI F, PAGLIACCI M C, MIGLIORATI G, MORACA R, GRIGANI F, RICCARDI C, NICOLETTI I (1994) The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells. LeukRes. 18 431-9. 

Example using a suspension cell line (Jurkat T-leukemia cell) (see Notes 3 and 4). 

The same group has also repotted a multicomponent Hantzsch protocol which provided access to polycyclic indenoheterocycles 78 utilizing a variety of aromatic and heterocyclic amines (Scheme 19) [45]. Some of the compounds disclosed showed apoptosis-inducing activity in a human T-cell leukemia cell line similar to etoposide. One of the most potent analogs was compound 79 (Scheme 19) which showed greater than 30% induction of apoptosis at 25 pM, as assessed in a flow cytometric aimexin V/propidium iodide assay in Jurkat cells. 

Jurkat human T cell leukemia cells (Jurkat cells, DSMZ Cat no. ACC 282). 

The Jurkat (human acute T-cell leukemia, clone E6-1) cell line was from ATCC. Cytochrome c ELISA ( MCTC0), active caspase-3 ELISA ( KM300), active caspase-7 ELISA ( KM700), bzVKD-fmk ( FMK011 or included with kits), human Bid ( 846-BD), mouse Bid ( 860-MM), caspase-8-cleaved human Bid ( 882-B8), caspase-8 cleaved mouse Bid ( 883-M8), mouse Bcl-xL ( 878-BC) missing the carboxyl terminal mitochondria targeting sequence, Bak-BH3 synthetic peptide ( 881-BA), Bak L to A synthetic peptide ( 879-BK), and HRP conjugated to streptavidin, recombinant human caspase 2 ( 702-C2), caspase 3 ( 707-C3), caspase 7 ( 823-C7), caspase 8 ( 705-C8), and caspase 10 ( 834-CP) were from R D Systems. Biotinylated standards for the ELISAs were made and assayed as previously... 

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