Reporter gene assays

Oestrogen receptor binding/Reporter gene assay. 

PIAS Proteins. Figure 1 Reporter gene assay with an interferon-y-driven artificial promoter demonstrating the inhibitory transcriptional activity of increasing amounts of PIAS on STAT1 signal transduction. 

Legler J, van den Brink C, Brouwer A, et al. 1998. Assessment of (anti-)estrogenic compounds using a stably transfected luciferase reporter gene assay in the human T47-D breast cancer cell line. Organohalogen Compounds 37 265. 

The yeast reporter gene assays not only assess for the interaction of the chemical with the hormone receptor, but also the ability of that receptor-chemical ligand interaction to activate the hormone DNA response element. It should be realized, however, that most of these systems have been developed with human and mammalian hormone receptors and differences in ligand potencies can occur between different animal species. A comprehensive review of in vitro assays for measuring estrogenic activity, and some of the issues of comparability, is provided by Zacharewski (1997). 

Cell based assays for NRs range from reporter gene assays to in vivo recruitment assays. The most reported of these is the GAL4 reporter assay. This assay takes advantage of the fact that the GAL4 response element of yeast does not exist in mammalian systems. 

Table 14.1 IL-2 reporter gene assay (transfected Jurkat T cells) activity of pateamine A and derivatives... 

Immunoassay, nucleic acid, and reporter gene assays... 

Schreurs, R., et al.. Estrogenic activity of UV filters determined by an in vitro reporter gene assay and an in vivo transgenic zebrafish assay. Arch. Toxicol, 76, 257-61, 2002. 

OECD. 2006e. Stably transfected transcriptional activation TA) assay for detecting estrogenic activity of chemicals - The human estrogen receptor alpha mediated reporter gene assay using HeLa-hER-9903 cell line. Version October 2006. Paris OECD, http //www.oecd.org/dataoecd/49/55/37531918.pdf... 

PXR Binding Reporter Gene Assays Scaled Down Heptocyte Induction Heptocyte Induction EC50/Emax (N=3)... 

El-Sankary, W Gibson, G.G., Ayrton, A. and Plant, N. (2001) Use ofa reporter gene assay to predict and rank the potency and efficacy of CYP3A4 inducers. Drug Metabolism and Disposition The Biological Fate of Chemicals, 29, 1499-1504. 

R. (2004) A PXR reporter gene assay in a stable cell culture system CYP3A4 and CYP2B6 induction by pesticides. Biochemical Pharmacology, 68,2347-2358. 

S. W. (2002) A cell-based reporter gene assay for determining induction of CYP3A4 in a high-volume system. Journal of Pharmacology and Experimental Therapeutics, 303, 412-423. 

Persson, KP., Ekehed, S., Otter, C., Lutz, E.S., McPheaL J., Masimirembwa, C.M. and Andersson, T.B. (2006) Evaluation of human liver slices and reporter gene assays as systems for predicting the cytochrome p450 induction potential of drugs in vivo in humans. Pharmaceutical Research, 23, 56-69. 

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