Isolation of genes

Young, R. A., and Davis, R. W., 1983. Efficient isolation of genes using and-body probes. Proceedings of the National Academy of Sciences U.S.A. 80 1194-1198. Using andbodies to screen protein expression libraries to isolate the structural gene for a specific protein. 

Isolation of genes. On the basis of prevention is better than cure, the process of identifying and isolating the affected gene in any inherited disorder almost... 

As this suggests, the major problem we face is the recognition and isolation of genes which confer stress tolerance. Thereafter, we may use reverse genetics to confirm the adaptive significance of these genes. Other approaches to gene isolation will now be discussed. 

Takenaka M et al. Isolation of genes identified in mouse renal proximal tubule by comparing different gene expression profiles. Kidney Int 1998 53 562— 572. 

The advent of recombinant DNA technology has allowed the isolation of genes and expression of proteins that are found in biologic tissues in exceedingly small quantities. This has permitted large-scale production of enzymes such as tissue plasminogen activator (i.e., tPA, alteplase) that cannot be extracted from tissues in quantities required for therapeutic use. Table 9.2... 

I) Isolation of genes and the preparation of cloned DNA. There are two general methods for obtaining cloned DNA ... 

The identification and isolation of genes involved in flavonoid biosynthesis has benefited from the fact that many of the flavonoids are colored compounds. Mutant phenotypes are therefore often easily identifiable based on variation in color. In Arabidopsis many of the genes involved in flavonoid bioysnthesis have been uncovered based on the change in seed coat (testa) color. Wild-type Arabidopsis seeds have a brown color, and mutations in flavonoid biosynthetic genes result in yellow or pale brown color because the underlying cotyledons are visible. These mutants are referred to as... 

Suppression-subtractive hybridization (SSH) is another hybridization strategy that is being pursued to examine differential gene expression following parasite infection in the snail host. At least two major laboratories are currently engaged in using this approach towards the isolation of genes that are specifically expressed in either resistant or susceptible snails in response to infection. Initial results from these studies showed differential expression of several transcripts in a resistant (BS-90) and susceptible snail (M-line) in response to... 

Bowman, E.J., Tenney, K., and Bowman, B. J. (1988). Isolation of genes encoding the. Xrurospora v.icuolar ATP.ise Analysis ofvma—1 encoding the 67-kDa subunit reveals homology to other ATPases./. Biol. Chem. 263, 13994-14001. 

Crameri R, Jaussi R, Menz G, Blaser K Display of expression products of cDNA libraries on phage surfaces. A versatile screening system for selective isolation of genes by specific gene-product/ligand interaction. Eur J Biochem 1994 226 53-58. 

Identification of activity and assay Isolation of gene or cDNA Appropriate expression and characterization DEVELOPMENT ... 

Isolation of genes of importance to therapeutic and diagnostic development. 

Phillips, S. M., Bendall, A. J. and Ramshaw, I. A. (1990). Isolation of gene associated with high metastatic potential in rat mammary adenocarcinomas. J. Natl. Cancer Inst. 82,199-203. 

Polymerase chain reaction is particularly useful for genetic analysis because both amplification and primer specific isolation of gene fragments occur simultaneously. Allele-specific amplification can be employed to detect a single base-pair mutation through the use of a specially designed primer which is complementary to... 

Dr. D.P. Kasbekar, CCMB, Hyderabad and Dr. Godeleine Faugeron, Institute Jacques Monod, Universite Paris VII, Paris on Isolation of Genes Encoding Sterol Biosynthetic Enzymes from Ascobolus immersus (ongoing)... 

Nasmyth, K. A., and S. I. Reed. 1980. Isolation of genes by complementation in yeast molecular cloning of a cell-cycle gene. Proc. Natl Acad. Sci. USA 77 2119-2123. 

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