Suppression subtractive hybridization

Matsumoto, N, Kitayama, H, Kitada, M, Kimura, K, Noda, M, and Ide, C, 2003. Isolation of a set of genes expressed in the choroid plexus of the mouse using suppression subtractive hybridization. Neuroscience 117,405-415. 

Kim M, Kim S, Kim S, Kim B-D (2001) Isolation of cDNA clones differentiaUy accumulated in the placenta of pungent pepper by suppression subtractive hybridization. Mol CeUs 11 ... 

Suppression-subtractive hybridization (SSH) is another hybridization strategy that is being pursued to examine differential gene expression following parasite infection in the snail host. At least two major laboratories are currently engaged in using this approach towards the isolation of genes that are specifically expressed in either resistant or susceptible snails in response to infection. Initial results from these studies showed differential expression of several transcripts in a resistant (BS-90) and susceptible snail (M-line) in response to... 

Nowak, T.S., Woodards, A.C., Jung, Y., Adema, C.M. and Loker, E.S. (2004) Identification of transcripts generated during the response of resistant Biomphalaria glabrata to Schistosoma mansoni infection using suppression subtractive hybridization. Journal of Parasitology 90, 1034-1040. 

Preliminary EST data fiom this study as well as suppression subtractive hybridization results have identified several promising gene candidates. In addition, we have recently... 

Bayne, C.J., L. Gerwick, K. Fujiki, M. Nakao and T. Yano. Immune-relevant (including acute phase) genes identified in the livers of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization. Dev. Comp. Immunol. 25 205-217, 2001. 

Diatchenko, L., Y.F. Lau, A.P. Campbell, A. Chenchik, F. Moqadam, B. Huang, S. Lukyanov, K. Lukyanov, N. Gurskaya, E.D. Sverdlov and P.D. Siebert. Suppression subtractive hybridization a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93 6025-6030, 1996. 

Sangrador-Vegas, A., J.B. Lennington and T. Smith. Molecular cloning of an IL-8-like CXC chemokine and tissue factor in rainbow trout (Oncorhynchus mykiss) by use of suppression subtractive hybridization. Cytokine 17 66-70, 2002. 

Girard, J.P. et al. (1999) Heterogeneity of endoffielial cells the specialized phenotype of human high endothelial venules characterized by suppression subtractive hybridization. American Journal of Pathology, 155, 2043-2055. 

LUO D, HU w, CHEN s, XIAO Y, SUN Y and ZHU z (2009) Identification of differentially expressed genes between cloned and zygote-developing zebrafish (Danio rerio) embryos at the dome stage using suppression subtractive hybridization. Biol Reprod, 80, 674-684. 

Dai Z,Mao X.MagnusonJK, Lasure LL. Identification of genes associated with morphology in Aspergillus niger by using suppression subtractive hybridization. Appl Environ Microbiol 2004 70 2474-85. 

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