Hybridization Strategies

For this hybridization strategy to work, the two probes (the capture and the labeled probe) should be from two adjacent portions on the genome, but without having complementary regions, thus avoiding binding of probes to each other. This hybridization requires target sample to bind to both the capture and the labeled probe and, as such, is more specific than direct hybridization on a membrane filter. 

Increased control of film composition, structure and size can be achieved by limiting the rate of reaction. This is possible using gas phase deposition where the amount of reactant is relatively low. Gas phase deposition loosely covers any hybridization strategy where at least one of the hybrid components is in the gas phase. This includes chemical vapor deposition (CVD), physical vapor deposition (PVD) and atomic layer deposition (ALD) as well as various plasma, sputtering and evaporation processes. 

Tab. 5.1 Comparison of nanocarbon hybridization strategies discussed within this chapter.

Suppression-subtractive hybridization (SSH) is another hybridization strategy that is being pursued to examine differential gene expression following parasite infection in the snail host. At least two major laboratories are currently engaged in using this approach towards the isolation of genes that are specifically expressed in either resistant or susceptible snails in response to infection. Initial results from these studies showed differential expression of several transcripts in a resistant (BS-90) and susceptible snail (M-line) in response to... 

Hybrid strategy for real time optimization with feasibility driven for a large scale three-phase catalytic slurry reactor... 

Hybrid Strategy for Real Time Optimization with Feasibility Driven for a Large Scale Three-Phase Catalytic Slurry Reactor... 

Actually the form of working equation of the first order cluster amplitudes and hence the perturbative scheme depend on the treatment of the four above-mentioned terms. One can treat all the four terms consistently in the same partitioning scheme. This approach is rather inflexible, since this necessarily constrains us to use only a very specific partitioning strategy, viz. the one proposed by Dyall [48]. We follow here a somewhat hybrid strategy where we treat the first and the second terms strictly by perturbation theory, while we treat the third and fourth terms on the same footing, but not in the sense of strict perturbation. Since it is natural to have the unperturbed state-energy Eq appear in the denominator in the RS version, we approximated Hij y in these terms by in since this leads to ... 

Several variants of the two-hybrid strategy have been developed. Some result solely from technical differences (e.g., use of different reporters, DNA-binding domains, fusion of the bait to the activation domain rather than to the DNA-binding domain), while others allow, for example, for... 

The pH of the reaction medium is another important factor for modulating both the activity and enantioselectivity of Lipase OF. The enzyme showed optimal hydrolysis activity at pH 4.0, while the enantioselectivity increased sharply with the decrease in medium pH from 4.0 to 2.2. Based on spectroscopic studies, the enhancement of the lipase activity and enantioselectivity at the lower pH could be attributed to the changes in the flexible and sensitive conformation of the lipase induced by tuning the biocatalyst microenvironment. Using a hybrid strategy by modulating pH and surfactant, enantiomer-enriched (5)-ketoprofen could be obtained with 95.5% ee and 39.1% yield from rac-ketoprofen chloroethyl ester (100 mM) at pH 2.5 in the presence of 0.5% (w/v) Tween-80 as a modulator. ... 

Brewer, M. J., Aitken, C. G. G. Talbot, M. 1996. A comparison of hybrid strategies for Gibbs sampling in mixed graphical models. Computational Statistics Data Analysis, 21(3) 343-365. 

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