Intracellular calcium, dynamics

BIOLUMINESCENCE IMAGING OF INTRACELLULAR CALCIUM DYNAMICS BY THE PHOTOPROTEIN OBELIN... 

Bioluminescence Imaging of Intracellular Calcium Dynamics by the Photoproteln Obelin 361... 

Building oscillations bottom up Elemental time scales of intracellular calcium dynamics... 

Li X et al (2007) Real-time monitoring of intracellular calcium dynamic mobilization of a single... 

Ventura, A. C., Bruno, L., and Dawson, S. P. (2006). Simple data-driven models of intracellular calcium dynamics with predictive power. Phys. Rev. E. Stat. Nonlin. Soft. Matter Phys. 74, 011917. 

Although in in vivo circumstances an intracellular free calcium increase apparently acts as the primary modulator of contraction, it can be bypassed in highly permeabilized smooth muscle preparations where the active subunit of MLCK can be introduced to phosphorylate myosin and induce contraction. The MLCK catalyzed phosphorylation of serine-19 is seen as the necessary event in the activation of smooth muscle myosin to form crossbridges. Thus, the rising phase of force during an isometric smooth muscle contraction follows an increase in the degree of phosphorylation of myosin, and that in turn follows the transient rise of (a) cytosolic free Ca, (b) Ca-calmodulin complexes, and (c) the active form of MLCK. The regulation of the intracellular calcium is discussed below. The dynam-... 

Rozov A, Burnashev N, Sakmann B, Neher E (2001) Transmitter release modulation by intracellular Ca2+ buffers in facilitating and depressing nerve terminals of pyramidal cells in layer 2/3 of the rat neocortex indicates a target cell-specific difference in presynaptic calcium dynamics. J Physiol 531 807-26... 

Fitzgerald JB, Jin M, Dean D et al. (2004) Mechanical compression of cartilage explants induces multiple time-dependent gene expression patterns and involves intracellular calcium and cyclic AMR J Biol Chemistry 279 19502-19511 Hunter CJ, Mouw JK, Levenston ME (2004) Dynamic compression of chondrocyte-seeded fibrin gels effects on matrix accumulation and mechanical stiffness. Osteoarthritis Cartilage 12 117-130... 

Calcium turnover in the bone is a highly dynamic process the bone serves as a reservoir for circulating and intracellular calcium. 

Changes in intracellular calcium homeostasis produced by active metabolites of xenobiotics may cause disruption of the dynamic cytoskeleton. There are a few toxins that cause disruption of the cytoskeleton through mechanisms independent of biotransformation. Microcystin is one of these toxins. Microcystin is produced by the cyanobacterium Microcystis aeruginosa. Similar toxins are produced by other species of cyanobacteria. The hepatocyte is the specific target of microcystin, which enters the cell through a bile-acid transporter. Microcystin covalently binds to serine/threonine protein phosphatase, leading to the hyperphosphorylation of cytoskeletal proteins and deformation of the cytoskeleton (Treinen-Moslen, 2001). 

Another proving ground for mathematical modeling in cell signaling has been the dynamics of intracellular calcium. Spurred by the nonlinear nature of the calcium release mechanisms, which exhibit cooperativity and both positive and negative feedbacks, and by the availability of fluorescent dyes for... 

Finally, tracking immature T cell (thymocyte) dynamics and morphology has also been combined with imaging the thymocyte calcium dynamics (Bhakta et al. 2005). Positive selection of thymocytes for maturation was correlated with an arrest of motility—a characteristic of a prolonged interaction—along with increased oscillatory intracellular calcium levels. 

Intracellular processes such as calcium dynamics and nuclear envelope breakdown... 

Single compartment models are desirable in this work, because any interaction between the mathematical models and the corresponding experimental systems should be based on the same known quantities. It would not be reasonable to include variables in the models that either cannot be determined from separate equations or cannot be provided as an input from the available experimental data. To check and validate models, we are using microscopy and imaging techniques to capture dynamic calcium events taking place within a cell as a whole, as opposed to calcium diffusion across a cell or intracellular calcium waves, for example (Patterson et al., 2007). 

Living cells visualization of membranes, lipids, proteins, DNA, RNA, surface antigens, surface glycoconjugates membrane dynamics membrane permeability membrane potential intracellular pH cytoplasmic calcium, sodium, chloride, proton concentration redox state enzyme activities cell-cell and cell-virus interactions membrane fusion endocytosis viability, cell cycle cytotoxic activity... 

Recent reviews pertaining to such topics as the role of calcium in growth control (82), lectins as natural membrane components (83), fibronectins (84), and biochemical studies of the cell cycle (85, 86, 87, 88), together with many of the works cited earlier, suggest a dynamic, time-dependent relationship between extracellular factors, membrane components and the intracellular metabolic machinery as a cell goes from... 

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