Interleukin-4 , transgenic expression

Barnum SR, Jones JL, Muller-Ladner U, Samimi A, Campbell IL (1996) Chronic complement C3 gene expression in the CNS of transgenic mice with astrocyte-targeted interleukin-6 expression. Glia 18 107-117. 

Bruder JT, Jie T, McVey DL, Kovesdi I (1997) Expression of gpl9K increases the persistence of transgene expression from an adenovirus vector in the mouse lung and liver. J Virol 71 7623-7628 Bruder JT, Kovesdi I (1997) Adenovirus infection stimulates the Raf/MAPK signaling pathway and induces interleukin-8 expression. J Virol 71 398-404 Burgert H-G (1996) Subversion of the MHC class I antigen presentation pathway by adenoviruses and herpes simplex viruses. Trends Microbiol 4 107-112... 

Peng, Y, Falck-Pedersen, E., and Elkon, K.B., Variation in adenovirus transgene expression between BALB/c and C57BL/6 mice is associated with differences in interleukin-12 and gamma interferon production and NK ceU activation, /. Virol, 75,4540,2001. 

Gutierrez-Ortega, A., Avila-Moreno, R, Saucedo-Arias, L.J., Sanches-Torres, C., and G6mez-Lim, M.A. (2004). Expression of a single-chain human interleukin-12 gene in transgenic tobacco plants and functional studies. Biotechnol. Bioeng. 85(7) 734-740. 

Friedlander, R. M., Gagliurdini, V., Hara, H., Fink, K. B., Li, W., MacDon-old, G., Fishman, M. C., Greenberg, A. H., Moskowitz, M. A., Yuan,J., (1997). Expression of a dominant negative mutant of interleukin-1 (3 converting enzyme in transgenic mice prevents neuronal cell death induced by trophic factor withdrawal and ischemic brain injury.. Exp. Med. 185, 933—940. 

Chan, L.S. Robinson, N., and Xu, L. Expression of interleukin-4 in the epidermis of transgenic mice results in a pruritic inflammatory skin disease an experimental animal model to study atopic dermatitis. J. Invest. Dermatol. 117, 977-983 (2001). 

Today, recombinant protein production involves many options. In addition to E. coli, several yeast systems (see Part IV, Chapter 13), insect cells (see Part IV, Chapter 14), different mammalian expression systems (CHO, BHK, NSO, HKBll, PER.C6) (see Part II, Chapter 3 and Part IV, Chapters 1 and 3) other alternative expression systems are currently under development for the production of biopharmaceuticals. These include transgenic animals or plants, and will be discussed in Part IV, Sub-Part 2 of this book. This chapter will focus on E. coli, a still-modern secretory Saccharomyces ccrevisiac system, and the recently developed mammalian HKBll expression system. An E. coli host/vector system is described that was originally developed for the efficient production of an interleukin-4 variant Later, it transpired that this system is ideally suited to the expression of other proteins and Fab fragments. The secretory... 

Powell, H.C., Garrett, R.S., Brett, F.M., Chiang, C.S., Chen, E., Masliah, E., and Campbell, I.L., Response of glia, mast cells and the blood-brain barrier, in transgenic mice expressing interleukin-3 in astrocytes, an experimental model for CNS demyelina-tion, Brain Pathol., 9(2), 219, 1999. 

Brett, F.M., Mizisin, A.P., Powell, H.C., and Campbell, I.L., Evolution of neuropatho-logic abnormalities associated with blood-brain barrier breakdown in transgenic mice expressing interleukin-6 in astrocytes, J. Neuropathol. Exp. Neurol., 54(6), 766, 1995. Chan, P.H., Epstein, C.J., Li, Y., Huang, T.T., Carlson, E., Kinouchi, H., Yang, G., Kamii,... 

Braat, H., Rottiers, R, Hommes, D. W., Huyghebaert, N., Remaut, E., Remon, J. R, et al. (2006). A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn s disease. Clinical Gastroenterology and Hepatology, 4, 754—759. 

Expression of interleukin-4 in nondliated epi-thehal cells in the conducting airways of transgenic mice resulted in a 6.5-fold increase in saturated phosphatidylcholine, a 15-fold in surfactant protein D in the alveolar pool relative to wild type mice (Ikegami et al. 2000). 

Similarly, in transgenic mice carrying the human interleukin-3 (IL-3) a or Pc receptor, these receptors are expressed not only in bone marrow cells, but also in the brain (pc receptor) and testis (a and Pc receptors) of the transgenic mice. Whether or not such expression is physiological is not yet known nevertheless these findings in transgenic mice may provide information about possible adverse effects in humans. 

Castelnau, P. A. Garrett, R. S. Palinski, W. et al. Abnormal iron deposition associated with lipid peroxidation in transgenic mice expressing interleukin-6 in the brain. 7. Neuropathol. Exp. Neurol. 

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