Lipid association with

M. M. Timbs and N. L. Thompson, Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery, Biophys. J. 58, 413-428 (1990). 

Nelson, D. R., Freeman, T. P., and Buckner, J. S. (2000). Waxes and lipids associated with the external waxy structures of nymphs and pupae of the giant whitefly, Aleurodicus dugesii. Comp. Biochem. Physiol. B 125,265-278. 

Vogel, J. J., Boyan-Salyers, B. D. Acidic lipids associated with the local mechanism of calcification. A review. Clin. Orthop. 118, 230 (1976)... 

Vasanthan, T. and Hoover, R. 1992. A comparative study of the composition of lipids associated with starch granules from various botanical sources. Food Chem. 43 19-22. 

The lipid has, more or less, the conformation shown in the diagram with all the polar ester groups at one end and the hydrocarbon chains bunched together in a nonpolar region. Oil and water do not mix, it is said, but triglyceride lipids associate with water in a special way. A drop of oil spreads out on water in a very thin layer. It does so because the ester groups sit inside the water and the hydrocarbon side chains stick out of the water and associate with each other. 

Whey protein concentrate. The whey protein used was prepared by ultrafiltration and spray drying. Protein content (N x 6.55) was 68% (dry weight). Lipid content was 7.1% (dry weight). In order to study heat induced aggregation by spectrophotometric methods the turbidity of the dilute protein dispersions was too high. The turbidity of whey protein dispersions is caused by lipids associated with proteins probably in the form of emulsified oil droplets. This fraction was removed by precipitation at pH 4.5 from dispersions made in dist. water and separated by centrifugation at 40 000 xg. 

GC-MS has been responsible for the identification of a variety of unusual lipids associated with diseased conditions. Hydroxyocta-decadienoic esters of cholesterol for example, have been isolated from aortal atheroma placques [277] and branched chain and odd numbered fatty acids identified in the glycerolipids of brain, spinal cord and sciatic nerve [278] from a patient with methylmalonic aciduria. The latter compounds are thought to arise by the replacement of malonyl CoA with methyl malonyl CoA, and acetyl CoA with propionyl CoA at certain stages of fatty acid synthesis. In these and other examples, the lipids need to be hydrolysed to permit the identification of the constituent fatty acids. As the class of lipids is usually known from the separation procedure used, the nature of the fatty acids may allow the characterisation of the complete molecule. However, volatilisation of the intact lipid into the mass spectrometer when possible would be preferable, particularly when it is present in a mixture and separation of the components is first made by GC. 

Aromatic hydrocarbons from bacteria Several species of bacteria have been shown to synthesise polycyclic aromatic hydrocarbons the anaerobic Clostridium putride assimilates lipids associated with dead plankton forming benzo(a)pyrene (Lima-Zanghi, 1968) Niaussat et al. (1970) observed that Bacillus badius synthesises benzo(a)pyrene and perylene. The hydrocarbon... 

The chlorophylls and carotenoids are the pigmented lipids associated with chloroplast membranes. Substituted pyridazinones reduce the accumulation of these pigments (Table II). 

Present in sulfoquinovosyldiacylglycerol, the lipid associated with photosynthesis. Important component of the biological sulfur cycle. 

Table 4. Increased concentrations of ether lipids associated with visceral diseases ...

Kelley (1964) found that proteolysis completely destroyed 20 percent of the lipoprotein molecules in a low density lipoprotein solution. The lipid from these molecules was transferred to the remaining lipoprotein molecules which were less stable during storage but resistant to proteolytic enzyme attack. The additional lipid associated with modified lipoproteins may explain the enhanced ether extractability observed previously. These experiments indicate that lipid-protein interactions contribute to the stability of the molecular complex. 

PG, MGDG and DGDG, lipids associated with the plastid membranes were richer... 

Terelitsky, M.J., and W.W. Barrow Postphagocytic Detection of Glycopeptido-lipids Associated with the Superficial Lj Layer of Mycobacterium intracellulare. Infect. Immun. 41, 1312 (1983). 

The influence of incubation temperature on liposome uptake by CVl cells was examined. The cells were ineubated with either the pol5mier-modified or the unmodified EYPC liposomes containing a fluorescent lipid, NBD-PE, at 37 or 42°C for 3 h. The amount of liposomal lipid associated with the cells after the incubation was evaluated from the fluorescence intensity of NBD-PE in the cells (Fig. 6). For the unmodified liposome, approximately the same amount of liposome was taken up by the cell at both temperatures, indicating that temperature does not affect the liposome uptake in this temperature region. In the case of the polymer-modified liposome, the amount of liposome taken up by the cell at 37°C was slightly lower than that of the unmodified liposome at the same temperature. However, at 42°C the amount became two times higher than that of the unmodified liposome. In... 

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