Impedimetric immunosensors

P. Cooreman, R. Thoelen, J. Manca, M. vande Ven, V. Vermeeren, L. Michiels, M. Ameloot, and P. Wagner, Impedimetric immunosensors based on die conjugated polymer PPV. Biosens. Bioelectron. 20, 2151-2156 (2005). 

O. Ouerghi, A. Touhami, N. Jaffrezic-Renault, C. Martelet, H. Ben Ouada, and S. Cosnier, Impedimetric immunosensor using avidin-biotin for antibody immobilization. Bioelectrochemistry 56, 131—133... 

S. Hleli, C. Martelet, A. Abdelghani, N. Burais and N. Jaffrezic-Renault, Atrazine analysis using an impedimetric immunosensor based on mixed biotinylated self-assembled monolayer, Sens. Actuators B Chem., 113(2) (2006) 711-717. 

Valera, E., J. Ramon-Azcon, A. Rodriguez, et al. 2007. Impedimetric immunosensor for atrazine detection using interdigitated [mu]-electrodes (ID[mu]E s). Sens. Actual B Chem. 125 526-537. 

Ramdn-Azcon, J., E. Valera, A. Rodriguez, et al. 2008. An impedimetric immunosensor based on inter-digitated microelectrodes (IDp-E) for the determination of atrazine residues in food samples. Biosens. Bioelectron. 23 1367-1373. 

Rodriguez, A., E. Valera, J. Ramon-Azcon, et al. 2008. Single frequency impedimetric immunosensor for atrazine detection. Sens. Actuat. B Chem. 129 921-928. 

Ionescu, R.E., N. Jaffrezic-Renault, L. Bouffier, et al. 2007. Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic. Biosens. Bioelectron. 23 549-555. 

Applications. The most common application of electrofunctional polymers is in the development of new materials in the (bio)sensors field [78]. For instance, a pyrrole-modified biotin allows successive attachment of avidin and biotin-labeled glucose oxidase, resulting in an efficient glucose biosensor [77,79]. Oureghi et al. [80] developed an impedimetric immunosensor for... 

Katz, E., Willner, I. (2003). Probing biomolecular interactions at conductive and semicon-ductive surfaces by impedance spectroscopy routes to impedimetric immunosensors, DNA-sensors, and enzyme biosensors. Electroanalysis 15, 913-947. 

Impedimetric immunosensors based on the conjugated polymer PPV. Biosens Bioelectron 2005 20(10) 2151-6. 

Although they have been successfully applied at the academic level, commercial devices are being developed [548,549]. Impedimetric immunosensors could potentially be used for qualitative purposes, such as, for example, in the detection of bacteria, pregnancy tests, allergy screening tests, etc [546]. 

Yang, G., Jin, W., Wu, L. et al. (2011) Development of an impedimetric immunosensor for the determination of 3-amino-2-oxazolidone residue in food samples. Anal. Chim. Acta, 706, 120-127. 

Joung, C.-K Kim, H.-N Im, H.-C. et al (2012) Ultra-sensitive detection of pathogenic microorganism using surface-engineered impedimetric immunosensor. Sens. Actuators, B, 161, 824-831. 

Li, Y Cheng, P, Gong, J. et al. (2012) Amperometric immunosensor for the detection of Escherichia coli 0157 H7 in food specimens Anal. Biochem., 421,227-233. Joung, C.-K Kim, H.-N Lim, M.-C. et al. (2013) A nanoporous membrane-based impedimetric immunosensor for label-free detection of pathogenic bacteria in whole milk. Biosens. Bioelectron., 44,210-215. 

Chai, C Lee, J. and Takhistov, P. (2010) Direct detection of the biological toxin in acidic environment by electrochemical impedimetric immunosensor Sensors, 10, 11414-11427. 

Hayat, A., Barthelmebs, L. and Marty, J.-L. (2012) Electrochemical impedimetric immunosensor for the detection of okadaic acid in mussel sample. Sens. Actuators, B, 171-172, 810-815. 

Hafaid, L, Chebil, S., Korri-Youssoufi, H. et al. (2010) Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole. Sens. Actuators, B, 144, 323-331. 

Another recent example reports a label-free electrochemical impedimetric immunosensor for the sensitive detection of metabolite of furaltadone, 5-morpholino-3-amino-2-oxazolidone (AMOZ), one of the most common nitrofuran antibacterial agents... 

Under the optimized conditions, the relative change in impedance was proportional to the logarithmic value of AMOZ concentrations in the range of 1.0 to 1.0 x 10 ng/ml (r = 0.9991) with the detection limit of l.Ong/ml. The impedimetric immunosensor was successfully applied to the determination of AMOZ in animal derived food samples, such as chicken muscle, pork, swine casing, shrimp, egg, and honey, with good agreement with the results obtained by HPLC-MS/MS [24],... 

An example of the selective detection of E. coli involved an impedimetric immunosensor developed using SAM-modified gold screen-printed electrodes (SPEs) [27]. In this... 

Impedimetric Immunosensors Using Interdigitated Array Microelectrodes... 

The same authors developed a much more robust label-free impedimetric immunosensor, based on the same IDpE arrays conveniently derivatized through covalent procedures to ensure reliability and to prevent nonspecific adsorption [42]. Atrazine-BSA was covalently immobilized on the surface of the IDpEs previously activated with (3-glycidoxypropyl)trimethoxysilane via the side chain amino groups of lysines or arginines with the epoxy groups on the device surface. Before, the gold electrodes were blocked... 

Figure 14.18 Nyquist plot of the impedance spectrum corresponding to the development of an impedimetric immunosensor for atrazine detection (a) Step I antigen immobilization and (b) Step II incubation step. The impedance spectra include three regions (i) diffusion control, (ii) first semicircle, and (Hi) second semicircle. From Ref [41] with permission from Elsevier...

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