Avidin using

To purify the SATA-modified (strept)avidin use gel filtration on a desalting column or dialyze against 0.1M sodium phosphate, 0.15M NaCl, pH 7.2, containing lOmM EDTA. At this point, the derivative is stable and may be stored under conditions which favor long-term (strept)avidin activity. 

HRP is also conjugated to (strept)avidin using the two step glutaraldehyde procedure and is used in this form in the Labeled... 

Ghenciu EG, Beckman EJ. Affinity extraction into carbon dioxide. 1. Extraction of avidin using a biotin-functional fluoroether surfactant. Ind Eng Chem Res 1997 36 5366-5370. 

In another study Bachas and coworkers have described two different postcolumn detection techniques for biotin and biocytin after HPLC separation based on fluorescence (116). One method involves avidin using 2,6-ANS (Fig. 10) as the fluorophore, while the other one relies on fiuoresceine covalently linked to streptavidin. The principle of these fluorescence detections has already been discussed. The HPLC separation was achieved on a C18 colunm using 0.1 M potassium phosphate buffer pH 7.0 with methanol (8 2) as mobile phase. The postcol-... 

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