Immunoassay food products analysis

L.H. Stanker, B. Watkins, M. Vanderlaan, R. Elhs, and J. Rajan, Analysis of heptachlor and related cyclodiene insecticides on food products, in Immunoassays for Trace Chemical Analysis, ed. M. Vanderlaan, L.H. Stanker, B.S. Watkins, and D.W. Roberts, American Chemical Society, Washington, DC, Chapter 12, pp. 108-123 (1991). 

Stanker LH, Watkins B, Vanderlaan M, et al. 1989. Analysis of heptachlor and related cyclodiene insecticides in food products. In Vanderlaan M, ed. ACS (American Chemical Society) Symposium Series, 451. Immunoassays for trace chemical analysis Monitoring toxic chemicals in humans, food and the environment Meeting, Honolulu, Hawaii, December 17-22. Washington, DC American Chemical Society, 108-123. 

Immunometric methods find increasingly wide applications owing to their superiority and continuous improvements. One of the major advantages of immunometric methods is that they provide consumers with early information (prior to food consumption) on possible health hazards (allergenic characteristics) of specific food products. The sensitivity of immunoassays is largely dependent on the type of a compound subject to analysis (up to lOpg/mL of the analyte). 

TABLE 13.1 Immunoassays Used in the Analysis of Food Products... 

Thermal treatment of proteins present in processed foods may have implications beyond food safety to the functionality of these proteins. For this purpose, specific antibodies raised against native and denatured proteins which could distinguish the two forms would enable one by immunoassay to determine the extent of protein denaturation in the product and its associated implication in safety and functionality of the finished food. Paraf (87) discusses this aspect in his review on monoclonal antibodies in the analysis of food proteins. The ability of IA s to distinguish native versus heat-denatured ovalbumin dependent on the assay protocol used. One can foresee this... 

The evaluation of a number of immunoassay diagnostic kits was undertaken to determine their usefulness in a regulatory analytical laboratory environment in the food, feed and pesticide areas. Four rapid enzyme immunoassay tests for the detection of aflatoxin residues at the 20 ppb level in animal feeds were compared to the official HPLC procedure. In the pesticide area, a commercial pentachlorophenol competitive inhibition assay for residues in water was investigated as to its applicability to poultry and pork liver matrices. In addition, an ELISA screening procedure for the herbicide fusilade was developed. Modifications were incorporated into the rapid immunoband 1-2 Test procedure for the detection of motile Salmonella in various food and animal feed products resulting in quicker analysis than the standard culture method. Also, a comparative evaluation of a Quik-Card Test for sulphamethazine drug residues in pork urine, liver and muscle tissue, is described. 

Although the use of CE in food analysis is limited compared with LC, many applications have been reported in recent years in the study of food proteins and peptides. It has been shown that the sensitivity provided by CE using LIE detection for bovine whey proteins is twice as good as that provided by UV detection, under the same separation conditions. LIF detection has also been used together with affinity interactions to enhance the detection sensitivity in CE. For instance, an immunoassay detection method has been described using a polyclonal antibody marked with fluorescein to determine lactoferrin. CE-LIF has also been applied to the determination of amines and amino acids in several samples such as wine and milk products. 

See also Bloassays Overview. Derivatizatlon of Analytes. Extraction Solvent Extraction Principles. Fluorescence Food Applications. Food and Nutritional Analysis Contaminants. Gas Chromatography Detectors Mass Spectrometry. Immunoassays Production of Antibodies. Immunoassays, Applications Food. Immunoassays, Techniques Enzyme Immunoassays. Liquid Chromatography Instrumentation Liquid Chromatography-Mass Spectrometry Food Applications. Sampling Theory. 

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Principles and Instrumentation. Carbohydrates Starch. Clinical Analysis Glucose. Extraction Solvent Extraction Principles. Food and Nutritional Analysis Dairy Products Oils and Fats. Gravimetry. Immunoassays,... 

See also-. Food and Nutritional Analysis Contaminants Meat and Meat Products Dairy Products. Hormones Steroids. Immunoassays, Techniques Radioimmunoassays Enzyme Immunoassays. Pesticides. Polymerase Chain Reaction. Toxins Mycotoxins Neurotoxins. 

See also Archaeometry and Antique Analysis Dating of Artifacts. Bioassays Ovenriew. Drug Metabolism Metabolite Isolation and Identification Isotope Studies. Fertilizers. Food and Nutritional Analysis Meat and Meat Products. Immunoassays, Techniques Radioimmunoassays. Isotope Dilution Analysis. Pesticides. Pharmaceutical Analysis Drug Purity Determination. Process Analysis Overview. Radiochemical Methods Pharmaceutical Applications. Water Analysis Industrial Effluents. 

See also Blood and Plasma. Clinical Analysis Glucose. Enzymes Immobilized Enzymes Enzyme-Based Electrodes Enzymes in Physiological Samples Industrial Products and Processes. Ethanol. Flow Injection Analysis Principles Detection Techniques. Food and Nutritional Analysis Alcoholic Beverages. Forensic Sciences Alcohol in Body Fluids. Immunoassays, Techniques Enzyme Immunoassays. Lipids Determination in Biological Fluids. Pesticides. Process Analysis Bioprocess Analysis. 

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