Immuno affinity chromatography

Tissue inhibitor of metalloproteins (from human blood plasma). Purified by immuno-affinity chromatography and gel filtration. [Cawstin et al. BJ 238 677 1986]. 

Thienpont LM, Verhaeghe PG, Van Brussel KA, De Leenheer AP. Estradiol-17 beta quantified in serum by isotope dilution-gas chromatography-mass spectrometry reversed-phase C18 high-performance liquid chromatography compared with immuno-affinity chromatography for sample pretreatment. Clin Chem 1988 34 2066-9. 

Antibodies, as well as antigens, can be used as ligands. This case is often referred to as immuno affinity chromatography. ... 

Specific DNA adducts are excreted into urine as modified bases and deoxynucleotides [121-123]. As endonucleases and glycosylases repair the damaged DNA, deoxynucleotides and bases respectively are liberated. Oligonucleotides and bulky DNA adducts like benzo[a]pyrene-deoxynucleotides or aflatoxin-Bj-Ny-dG are also excreted. Immuno-affinity chromatography, GC/MS, LC-MS/MS,or HPLE-EC can be used to quantify the wide range of base modifications and thus to estimate the DNA repair activity, whereas ELISA assays do not yet have sufficient proven specificity. 

Birnbaum, S. (1992) Latex-based thin-layer immuno-affinity chromatography for quatita-tion of protein analytes, Analytical Biochemistry, 206,168-171... 

This paper reviews the present status of affinity separation of cells based on the biospecific interaction of cellular receptors with proteinaceous ligands immobilized on a solid-phase matrix. Special emphasis was placed on the development of new matrix materials for immuno-affinity chromatography of lymphocyte subpopulations. Our newly developed matrix of poly(2-hydroxyethyl methacrylate)/polyamine graft copolymer offered novel advantages in (1) elimination of non-specific adsorption of lymphocytes and (2) simple immobilization procedure of ligand protein through non-covalent adsorption. This matrix allowed a rapid separation of preparative quantities of pure and vital lymphocyte subpopulations (IgG-positive and -negative cells) in excellent yield. 

As will be described below, the first application of affinity chromatography in cell separation, reported in 1969 (14,15), was based on antigen-antibody reactions (immuno-affinity chromatography). Cellular immuno-affinity chromatography has since been further extended by a number of investigators, and in its present form, offers a wider applicability because of an increased number and variety of available antibodies. 

This paper briefly reviews the progress and present status of cell affinity selection with special emphasis on immuno-affinity chromatography, and then deals with our own studies on the development of new matrix materials specially designed for cell affinity chromatography. 

Application of Immuno-affinity Chromatography in Cell Separation In 1969, Wigzell et al (14) and Evans et al (J 5) first applied... 

Katz SE, Siewierski M, Drug residue analysis using immuno affinity chromatography, J. Chromatogr A... 

Vaks B, Mory Y, Pederson JU et al. (1984) A semi-continuous process for the production of human interferon- ac from E. coli using tangential-flow microfiltration and immuno-affinity chromatography. Biotechnol Lett 6(10) 621-626... 

Nachman M, Azad ARM, Bailon P. Efficient recovery of recombinant proteins using membrane-based immuno-affinity chromatography (MIC). Biotech. Bioeng. 1992 40 564-571. 

Automated systems for size-exclusion, or gel permeation, chromatography are very popular, particularly in pesticide residue analyses. Another form of column chromatography, based on the use of specific antibodies to the analyte(s) of interest, is immuno-affinity chromatography (lAC). In this technique, antibodies specific for one or more analytes are covalently bound to an activated support such as Se-pharose, which is then packed in a column (Figure 1). This technique is particularly suited for coupling... 

Hage DS and Kao PC (1991) High-performance immuno-affinity chromatography and chemiluminescent detection... 

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