Immune response genes mouse

The cells are cultured and in vitro restimulated with antigen (here PT) for 12 h to mimic the encounter with the specific antigen in the body. Earlier studies have shown that an in vitro restimulation of mouse spleen lymphocytes with antigen for 4 and 24 h, can be used to study immune response as well as other cellular processes, such as apoptosis, inflammation, and so on (see Chapter 16). Our experiments, however, showed that immune response markers are expressed at later time-points as compared to mouse, and that 12 h of in vitro restimulation is the ideal time-point for the assessment of immune response genes (see Table 17.1) (see Note 1). 

Changes in the Expression of Genes Involved in Growth and the Immune Response in Mouse Brains Expressing Constitutively Active a1B-Adrenergic Receptors... 

Class II MHC antigens are found only on certain lymphoid cells. These are products of (mouse) la and Ir (for immune response) genes. 

A recent paper clearly highlighted the limitations of in vitro systems in modeling whole-organism responses, which should be considered when developing biomarkers of in vivo toxicity. Dere and colleagues (58) compared the temporal gene expression profiles of Hepalclc mouse hepatoma cells and of the mice liver after treatment with a dioxin. The analysis revealed that Hepalclc cells were able to model the induction of xenobiotic metabolism in vivo. On the other hand, responses associated with cell cycle progression and proliferation were unique to the in vitro system, while lipid metabolism and immune responses were not replicated effectively in the Hepalclc cells. 

Cao H, Urban JF. Jr., Anderson RA. 2008. Cinnamon polyphenol extract affects immune responses by regulating anti- and proinflammatory and glucose transporter gene expression in mouse macrophages. J Nutr 138 833-840. 

Baekelandt V, Eggermont K, Michiels M, Nuttin B, Debyser Z (2003) Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain. Gene Ther 10 1933-1940. 

Michou AI, Santoro L, Christ M, Julhard V, Pavirani A, Mehtali M (1997) Adenovirus-mediated gene transfer Influence of transgene, mouse strain and type of immune response on persistence of transgene expression. Gene Ther 4 473 82. 

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