Mouse spleen lymphocytes

The cells are cultured and in vitro restimulated with antigen (here PT) for 12 h to mimic the encounter with the specific antigen in the body. Earlier studies have shown that an in vitro restimulation of mouse spleen lymphocytes with antigen for 4 and 24 h, can be used to study immune response as well as other cellular processes, such as apoptosis, inflammation, and so on (see Chapter 16). Our experiments, however, showed that immune response markers are expressed at later time-points as compared to mouse, and that 12 h of in vitro restimulation is the ideal time-point for the assessment of immune response genes (see Table 17.1) (see Note 1). 

None of the three isomers of xylene induced micronuclei or chromosomal aberrations in mouse bone marrow in vivo (chromosomal aberrations were observed in mouse spleen lymphocytes in vivo). 

Streptazolin (1) shows limited antibacterial and fungicidal activity and influences the incorporation of thymidine in mouse spleen lymphocytes. 

P-Epoxyambelline (cavinine) (372) has been found to be an immu-nostimulant, and it produces moderate activation of mouse spleen lymphocytes (55). Although the epoxy alkaloid undulatine (371) was inactive in 9 PS and 3 PS screens, ambelline (368) did exhibit in vitro activity in the 9 PS bioassay (5). 

Otani, H. and Hata, I. 1995. Inhibition of proliferative responses of mouse spleen lymphocytes and rabbit Peyer s patch cells by bovine milk caseins and their digests. J. Dairy Res. 62, 339-348. 

Mammalian cells Mouse (spleen lymphocytes) Chromosomal aberrations + Rithidech et al. 1987... 

Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Human (occupational exposure/lymphocytes) Mouse (spleen lymphocytes)... 

Mouse (spleen lymphocytes) RNA synthesis inhibition No data + Post et al. 1985... 

The identification of mouse spleen lymphocytes (B T cells) by SEM and laboratory light microscopy using immunolatex spheres as specific markers. 

Gene mutation, hprt locus, CD-I mouse spleen lymphocytes in vivo 1000 mg/kg bw/d, po 5 d/week, 3 weeks [220]... 

Penststemon serrulaftts Penstemide Aotitu moral Antiproliferative activity on mouse spleen lymphocytes and hepatoma cells in the Syrian hamster. Active [79]... 

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