Rabbit IgG

Bovine senun albumin y-Globulin Fibrinogen Rabbit anti-mouse Thymocyte IgG Concavalin A Human serum albumin BK 19.45 IgG BK 19.9 IgG Rabbit IgG (polyclonal) (carrier free)... 

Fig. 5. (a) Layout of a slide that was spotted and immobilized using the anti goat IgG rabbit antibody and the anti rabbit IgG goat-antibody, (b) Fluorescent image after incubation of a Cy5-labeled goat IgG. (c) Fluorescent image after incubation of a Cy5-labeled rabbit IgG. 

IgG (rabbit) Competitive immunoassay capture of analyte by sol-gel carbon ink incorporating IgG Amperometric detection of ALP-generated a-naphthol +400 mV 50-5000 ngml-1 5 ngmU1 (32 pM) Wang et al. [78]... 

IgG (rabbit) Homogeneous immunoassay competition between free and GOD-labelled IgG for surface-bound antibody Amperometric detection of H2O2 produced from glucose channelled through surface conducting polymer-bonded HRP -0.35 V 0.5-2.0 [xgml-1 0.33 [xgml-1 Darain et al. [79]... 

IgG (rabbit) Competitive immunoassay using ALP-labelled antibody at streptavidin-modified SPCEs bearing capture antibody Cyclic voltammetry of indigo carmine generated from 3-IP substrate —0.15 V (scan from —0.3 to +0.2 V) 5 x KT11 to 1 x 1(T9M 5 x 10 nM (7-Ongmr1) Diaz-Gonzalez et al. [83]... 

IgG (rabbit) Sandwich immunoassay upon Os redox hydrogel/ avidin-modified SPCE Amperometric HRP-mediated electrocatalytic reduction of H2O2 +0.2 V lpgml-1 to lOngml-1 7 pgml-1 Zhang and Heller [85]... 

Rabbit ala3/b4 immunoglobulin IgG Rabbit immunoglobulin anti-arginase antibody Rabbit immunoglobulin Ig anti-(D-glucose oxidase) antibody Rabbit immunoglobulin Ig anti-(human a-foeto-protein) antibody... 

Figure 2. Binding of [ IJpalytoxin to anti-palytoxin (Rabbit 633) before ( ) and after primary (o), first boost (A), second boost (A), and third boost ( , Rabbit 633D-24) of immunization and after absorption of IgG in Rabbit 633D-24 with goat anti-rabbit IgG ( ).

Kentolysin Compared to Heliantholysin. Stoichactis helianthus occurs in the Caribbean region whereas another species, Stoichactis kenti is distributed in the Indo-Pacific area. The latter produces a toxin, kentolysin, that is similar to, but not identical with heliantholysin (6). The amino acid compositions of the two polypeptides show a distinct resemblance but appear to differ significantly in number of residues of lysine, methionine, tyrosine and histidine. IgG from a rabbit immunized against heliantholysin neutralizes both heliantholysin and kentolysin, but neutralization of the homologous toxin is more efficient (Table III). It can be seen that in the concentrations used, the IgG failed to neutralize the related lytic peptides of Condylactis gigantea and Epiactis prolifera. 

Secondary antibody and determination. A secondary antibody labeled with an enzyme is added which binds to the primary antibody that is bound to the coating antigen. If the primary antibody were produced in a rabbit, an appropriate secondary antibody would be goat anti-rabbit immunoglobulin G (IgG) conjugated with horseradish peroxidase (HRP) (or another enzyme label). Excess secondary antibody is washed away. An appropriate substrate solution is added that will produce a colored or fluorescent product after enzymatic conversion. The amount of enzyme product formed is directly proportional to the amount of first antibody bound to the coating antigen on the plate and is inversely proportional to the amount of analyte in the standards. 

Smith, T.W. Lloyd, B.L. Spicer, N. and Haber, E. Immuno-genicity and kinetics of distribution and elimination of sheep digoxin-specific IgG and Fab fragments in the rabbit and baboon. Clin Exp Immunol 36 384-396, 1979. 

Figure 1.64 SATA modification of rabbit polyclonal IgG with the resultant sulfhydryl incorporation level.

The most common carrier proteins in use today are keyhole limpet hemocyanin (KLH MW 4.5 X 105 to 1.3 X 107), BSA (MW 67,000), aminoethylated (or cationized) BSA (cBSA), thyroglobulin (MW 660,000), ovalbumin (OVA MW 43,000), and various toxoid proteins, including tetanus toxoid and diphtheria toxoid. Other proteins occasionally used include myoglobin, rabbit serum albumin, immunoglobulin molecules (particularly IgG) from bovine or mouse sera, tuberculin purified protein derivative, and synthetic polypeptides such as poly-L-lysine and poly-L-glutamic acid. 

Geoghegan, W.D. (1988) The effect of three variables on adsorption of rabbit IgG to colloidal gold. J. Histochem. Cytochem. >6, 401-407. 

Hamaguchi, Y., Yoshitake, S., Ishikawa, E., Endo, Y., and Ohtaki, S. (1979) Improved procedure for the conjugation of rabbit IgG and Fab antibodies with b-D-galactosidase from Escherichia coli using N,N -o-phenylenedimaleimide. J. Biochem. (Tokyo) 85, 1289-1300. 

FIGURE 5.5 Schematic representations of the two immunosensor formats (a) immunosensor based on the biotin-streptavidin interaction and (b) immunosensor based on rabbit IgG-modified SPCEs. (Reprinted from [27] with permission from Elsevier.)... 

M. Diaz-Gonzalez, D. Hemandez-Santos, M.B. Gonzalez-Garcia, and A. Costa-Garcia, Development of an immunosensor for the determination of rabbit IgG using streptavidin modified screen-printed carbon... 

F. Darain, S.-U. Park, and Y.-B. Shim, Disposable amperometric immunosensor system for rabbit IgG using a conducting polymer modified screen-printed electrode. Biosens. Bioelectron. 18, 773—780 (2003). 

No antibody activity was found after oral immunization in any of the individual rabbits immunized with liposphere R32NS 1-vaccine formulation. However, rabbit immunization by all parenteral routes tested resulted in enhanced immunogenicity, with increased antibody IgG levels over the entire postimmunization period. The individual rabbit immune response shows that immunization by subcutaneous injection was the most effective vaccination route among all parenteral routes of administration tested. 

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