Hydrogels scaffolding

Nowak A.P., Breedveld V., Pakstis L., Ozbas B., Pine D.J., Pochan D., and Deming T.J. Rapidly recovering hydrogel scaffolds from self-assembling diblock copolypeptide amphiphiles. Nature, 417, 424, 2002. 

Song, J., Saiz, E. and Bertozzi, C.R. (2003) A new approach to mineralization of biocompatible hydrogel scaffolds an efficient process toward 3-dimensional bonelike composites. Journal of the American Chemical Society, 125, 1236-1243. 

In an attempt to mimic the physiological ratio of collagen type II to hyaluronan in the healthy human NP, Calderon et al. constructed hydrogels scaffolds composed of these two elements in a 9 1 (w/w) ratio [104]. Scaffolds were crosslinked with various concentrations of EDC/NHS, but it was found that 8 mM EDC/NHS resulted in a confined compressive modulus on the order of the native NP, while allowing for optimal rat mesenchymal stem cell (rMSC) viability and proliferation. Additionally, real time PCR results from rMSCs seeded on the scaffolds for 21 days indicated that the scaffolds promoted increased aggrecan expression and inhibited collagen type I expression compared to rMSCs cultured on monolayers. 

Fig. 3 Image frames captured from a video of an EGC hydrogel scaffold (a) prior to, (b) during, and (c) immediately following compression, illustrating its elastic shape-memory properties...

Fig. 4 Insert Representative macroscopic image of an APNP hydrogel scaffold seeded with human adipose-derived stem cells following 7 days of culture in a 12-well plate. Graph Osmotic swelling pressure profile of APNP hydrogel scaffolds (green-squares) compared to human NP (orange-triangles and red-diamonds) values found in the literature [28]...

Zhu J, Tang C, Kottke-Marchant K et al (2009) Design and synthesis of biomimetic hydrogel scaffolds with controlled organization of cyclic RGD peptides. Bioconjug Chem 20(2) 333-339... 

Chen FM, Zhao YM, Sun HH, Jin T, Wang QT, Zhou W, Wu ZF, Jin Y (2007) Novel gly-cidyl methacrylated dextran (dex-gma)/gelatin hydrogel scaffolds containing microspheres loaded with bone morphogenetic proteins formulation and characteristics. J Control Release 118(1) 65—77... 

Dillon GP, Yu X, Sridharan A, Ranieri JP, Bellamkonda RV. The influence of physical structure and charge on neurite extension in a 3D hydrogel scaffold. / Biomater Sci Polym Ed 1998 9 1049-69. 

Chen YM, Shen KC, Gong JP, Osada Y (2007) Selective ceU spreading, proliferation, and orientation on micropattemed gel surfaces. J Nanosci Nanotechnol 7 773 Chen YM, Tanaka M, Gong JP, Yasuda K, Yamamoto S, Shimomura M, Osada Y (2007) Platelet adhesion to human umbilical vein endothelial ceUs cultured on anionic hydrogel scaffolds. Biomaterials 28 1752-1760... 

Nowak, A.P. et al. Rapidly recovering hydrogel scaffolds from self-assembling diblock copolypeptide amphiphiles. Nature, 2002,417 424-8. 

Park, S. A., Lee, S. H., Kim, W. Fabrication of hydrogel scaffolds using rapid protot rping for soft tissue engineering. Macromol. Res.. 2011,19, 694-698. 

C.W. Chen, M.W. Betz, J.P. Fisher, A.B.S. Paek, Y. Chen, Macropo-rous hydrogel scaffolds and their characterization by optical coherence tomography. Tissue Eng. Part C Methods 17 (2011) 101-112. 

D.A. Young, D.O. Ibrahim, D. Hu, K.L. Christman, Injectable hydrogel scaffold from decellularized human lipoaspirate, Acta Biomater. 7 (2011) 1040-1049. 

Almany, L., Seliktar, D. Biosynthetic hydrogel scaffolds made from fibrinogen and polyethylene glycol for 3D cell cultures. Biomaterials 26, 2467-2477 (2005)... 

Andreopoulos, F.M. Persaud, I. 2006, Delivery of basic fibroblast growth factor (bFGF) from photoresponsive hydrogel scaffolds . Biomaterials, vol. 27, no. 11, pp. 2468-2476. 

Fisher JP, Jo S, Mikos AG, Reddi AH. Thermoreversihle hydrogel scaffolds for articular cartilage engineering. J Biomed Mater Res A 2004 71(2) 268—274. 

Cho, E. C., J. W. Kim, et al. (2007). Highly responsive hydrogel scaffolds formed by three-dimensional organization of microgel nanoparticles. Nano Letters 8(1) 168-172. 

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