Hybrid cell line

McLawhon, R.W. West, R.E., Jr. Miller, R.J. and Dawson, G. Distinct high-aff1nity binding sites for benzomorphan drugs and enkephalin in a neuroblastoma-brain hybrid cell line. Proc Natl MM USA, 78 4309-4313, 1981. 

Abstract. The hybrid cell lines, which we have obtained, can be widely used in veterinary virology and biotechnology for preparing vaccines, test-systems for viruses. Any strains of hybrid cells are producing the biological active proteins (enzymes and others). We have obtained hybrid cell lines (PO-TKxCO, PO-TKxHO), which are sensitive to prion protein, and also hybrid culture with P-cells of the pancreas of rabbit. 

Flow cytometry has been applied in many creative ways to the science of molecular biology. Flow sorting, based on Floechst 33258 and chromomycin A3 fluorescence, turns out to be one of the best ways available for obtaining relatively pure preparations of each type of chromosome. Even those chromosomes that are not distinguishable by their fluorescence (e.g., 9-12) can usually be sorted from hamster-human hybrid cell lines. These preparations of reasonably... 

Cho MS, Yee H, Chan S (2002), Establishment of a human somatic hybrid cell line for recombinant protein production, J. Biomed. Sci. 9 631-638. 

Klee WA, Nirenberg M. A neuroblastoma times glioma hybrid cell line with morphine receptors. Proc Natl Acad Sci USA 1974 71 3474-3477. 

Enzymatic N-demethylation of morphine also occurs in other opioid target tissues such as the GPI and hybrid cell lines but is absent in non-target tissue such as the diaphragm muscle. 

Fig. 1. Pertussis toxin-mediated ADP ribosylation of membrane G proteins. Isolated cell membranes (50 ng of protein) from N1E 115 cells (mouse neuroblastoma cell line), N2A cells (mouse neuroblastoma cell line), S49-1 eye cells (S49(-) mutated mouse lymphoma cell line deficient in Ga ), 549 wt cells (wild-type mouse lymphoma cell line), RBL (RBL 2H3 rat basophilic leukemia cell line), GH3 cells (GH3 rat hypophyseal tumor cell line), PC-12 (rat pheochromocytoma cell line), HIT-T15 cells (hamster insulinoma cell line), Y-1 cells (mouse adrenal cortex tumor cell line), 108 cc 15 cells (mouse/rat neuroblastoma x glioma hybrid cell line), HL-60 cells (DMSO-differentiated human leukemia cell line), HL-60 (+PT) cells (HL-60 cells pretreated with 25 ng/ml of pertussis toxin for 24 h prior to preparation of membranes), RINm5F cells (rat insulinoma cell line), and C6-2 cells (rat glioma cell line) were subjected to P-ADP-ribosylation as described in section 4.3.3. Samples were precipitated as outlined in section 4.3.5 and subjected to SDS-PAGE with separating gels containing 8% acrylamide (w/v). An autoradiogram of the dried gel is shown. Molecular masses of marker proteins are indicated (kDa). Modified Ga proteins migrate at approximately 40 kDa. Radioactivity running in front of the 30 kDa marker protein comigrates with the dye front...

Hybridomas (or fused cell culture lines) are produced by fusing lymphocytes isolated from the spleen of an immunized animal with the HPRT deficient malignant plasma cells. The lymphocytes. The unfused cells die, since spleen cells survive only a few days, while the malignant plasma cells lack HPRT. Fused cells, however, are HPRT positive as well as immortal these hybridomas, or hybrid cells, retain the Ab-producing characteristics of the spleen cells. Hybrid cell lines that produce a specific antibody are then cloned from the single cells and cultured. Each clone created in this manner produces antibodies of a single epitope specificity ... 

Elsayed et al. (1992) performed subcutaneous injections of GEES that resulted in increased GST activity, increased lipid peroxides, and depleted, reduced GSH in lung tissue (Elsayed et al., 1992). Similar findings occurred in the in vitro mouse model, a neuroblastoma-rat glioma hybrid cell line, in that there was a reduction in GSH as a function of time after exposure (Moore and Ray, 1983). Within the last 5 years, there has been a greater emphasis on the inhalation effects of SM. 

V. T. Oi, L. A. Herzenberg, in B. B. Mishell and S. M. Shiigi (Eds), Selected methods in cellular immunology. Immunoglobulin-producing hybrid cell lines, WH Freeman and Company, San Francisco, 1979, pp. 351-372. 

Robertson J, Raju M. 1980. Sudden reversion to normal radiosensitivity to the effects of x-irradiation and plutonium-238 alpha particles by a radioresistant rat-mouse hybrid cell line. Radiat Res 83 197-204. 

MacDermot, J., Blair, I.A. and Cresp, T.M. (1981). Prostacyclin receptors of a neuronal hybrid cell line. Divalent cations and ligand-receptor coupling. Biochem. Pharmacol, 30, 2041-2044... 

Leigh, P.J. and MacDermot, J. (1985). Desensitization of prostacyclin responsiveness in a neuronal hybrid cell line selective loss of high affinity receptors. Br. J. Pharmacol, 85, 237-247... 

All processed pseudogenes studied to date are located on different chromosomes from those of their functional counterparts (191). With previously characterized human-hamster hybrid cell lines, the ceruloplasmin pseudogene has been mapped to chromosome 8 (194). This differs from the assignment of the functional ceruloplasmin gene to chromosome 3 (87,182). 

Table 2. Mengovinis yield in hybrid cell lines (PPU/oell)...

Houghton AN, Brooks H, Cote RJ, Taormina MC, Oettgen HF, Old LJ (1983) Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derivedfrom lymphocytes of patients with malignant melanoma. J Exp Med 158 53-65... 

II. Lactate dehydrogenase and )8-glucuronidase. Genetics 54, 1111-1122. Weiss, M. C., and Green, H. (1967). Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc. Natl. Acad. Set. U.S. 58, 1104-1111. 

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