Epithelial hybrid cell line

Immunocytochemistry and in situ hybridization techniques were used to detect HIV-1 infected cells in the testis (P5), excurrent ducts, and prostate. Distinct pathologic changes were observed in the majority of testis of AIDS patients that included azoospermia, hyalinization of the boundary wall of seminiferous tubules, and lymphocytic infiltration of the interstitium. In the testis, many white blood cells were shown to the CD4 + HIV-1 positive cells of lymphocy-tic/monocytic morphology, found in the seminiferous tubules and interstitium of the testis, epididymal epithelium, and connective tissue of the epididymis and prostate. There was no evidence of active HIV-1 infection in germ cells or Sertoli cells of the seminiferous tubules or other epithelial cells lining the excurrent ducts or prostatic glands. 

Fig. 2. Resultant scan from a cDNA microarray analysis of Stat3-specific gene targets in mammary epithelial cells. (A) Portion of a 15,000 mouse cDNA array, hybridized with Cy3 (inactive Stat3) and Cy5 (active Stat3)-labeled cDNA probes from a mouse mammary epithelial cell line. Note the range of hybridization intensities between cDNAs spots on the array, representing relative differences in the expression of these transcripts in the complex probe populations. (B) and (C) Differential hybridization to individual cDNAs on the same part of the array is evident in separate scans of Cy-3 (B) and Cy-5 (C) labeled probes. These cDNA clones therefore represent likely gene targets of the Stat3 transcription factor.

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