HPLC-Mass Spectrometry

Chirinos, R. et al., High-performance liquid chromatography with photodiode array detection (HPLC-DAD)/HPLC-mass spectrometry (MS) profiling of anthocyanins from Andean mashua tubers (Tropaeolum tuberosum Ruiz and Pavon) and their contribution to the overall antioxidant activity, J. Agric. Food Chem., 54, 7089, 2006. 

Coldham, N. G. Woodward, M. J. Characterization of the Salmonella typhimurium proteome by semi-automated two dimensional HPLC-mass spectrometry Detection of proteins implicated in multiple antibiotic resistance. J. Proteome Res. 2004, 3,595-603. 

Salm, P. et al. 2005. A rapid HPLC mass spectrometry cyclosporine method suitable for current monitoring practices. Clin Biochem. 38 667. 

HPLC-mass spectrometry (MS). MS conditions ionization mode, atmospheric pressure ionization-electrospray (API-ES) polarity, positive Vcap, 4000 V nebulizer pressure, 35 psig drying gas, 10 L min gas temperature, 350 °C fragmentor, 70 V scan range, 120-600 atm. 

Characterization of Glucosinolates by HPLC-Mass Spectrometry Using Precursor Ion Scanning... 

Selection-independent analysis In this case, library analysis occurs strictly after and apart from the library selection experiment. Typically, what this means is that the solution resulting from a library is analyzed by HPLC or HPLC-mass spectrometry (HPLC-MS), and compared with the chromatographic trace obtained for an identical library prepared in the absence of target. This provides an internal control for self-selection processes and (hopefully) allows direct identification library members undergoing enhancement through visual inspection. If selfselection is the goal, one simply compares HPLC traces of libraries at different time points. 

Schmid, D., Behnke, B., Metzger, J., and Kuhn, R., Nano-HPLC-mass spectrometry and MEKC for the analysis of oligosaccharides from human milk, Biomedical Chromatography 16(2), 151-156, 2002. 

Barnes, S., Kirk, M., and Coward, L., Isoflavones and their conjugates in soy foods extraction conditions and analysis by HPLC-mass spectrometry, J. Agric. Food Chem., 42, 2466, 1994. 

Numerous papers have relied on only UV-visible spectra for their identification of phenolics, but for positive identification purposes, HPLC-mass spectrometry (MS) is another detection mode that can provide detection of all phenolic compounds in foods. This technique involves a hyphenated instrument that uses a mass spectrometer as a detector for HPLC or uses HPLC as cleanup step for mass spectrometry. After preparative HPLC, the MS technique has frequently been employed for structural identification of phenolics in many foods and essential oils because of its sensitivity and selectivity and its ability to provide structural information. 

Barnes S, Coward L, Kirk M, Sfakianos J. 1998. HPLC-mass spectrometry analysis of isoflavones. Proc Soc Exp Biol Med 217 254-262. 

Zeng, L. Kassel, D. B. 1998. Developments of a fully automated parallel hplc/mass spectrometry system for the analytical characterization and... 

Taylor PJ, Brown SR, Cooper DP, Salm P, Morris MR, Pillans PI, Lynch S V (2005) Evaluation of 3 internal standards for the measurement of cyclosporine by HPLC-mass spectrometry. Clin Chem 51 1890-1893... 

M. D. del Castillo, M. L. Sanz, M. J. Vicente-Arana, and N. Corzo, Study of 2-furoyl-methyl amino acids in processed foods by HPLC-mass spectrometry, Food Chem., 2002, 79, 261-266. 

In an interesting experimental protocol, Silvestro et al. (1993) utilized HPLC-mass spectrometry with an ion spray (electrospray) interface for determination of PAF and lysoPAF in human PMN (neutrophils). Both unstimulated and stimulated (with complement-activated zymosan) cells were used as starting material. The total lipids were isolated in the usual way, and the PAF was isolated and purified by a combination of thin-layer chromatography, HPLC, and silica chromatography. This final PAF preparation was subjected to a bioassay with the inclusion of 3H 16 0 PAF to monitor recoveries. 

Keywords Carotenoids analysis sample preparation UV/Visible spectroscopy geometrical isomers optical isomers HPLC mass spectrometry nuclear magnetic resonance metabolites. 

However, 0.5 g p-thiocresol and 0.5 mL m-cresol were used as scavengers as recommended in the literature for chloroacetylated peptide compounds (33). The crude peptide was purified by RP-HPLC and, after lyophilization, was characterized by analytical HPLC, mass spectrometry, and amino acid analysis. The pure product was obtained at 65% yield. 

---