Chromatography HPLC mass spectrometry

In species where pheromones are not typical hormones, intensive chemical analyses have been productive in search of pheromone compounds. Such analyses usually involve exhaustive chromatographic and spectromefric methods including high performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) to concentrate, purify and identify compounds in water conditioned by mature animals, and to confirm the pheromone functions through EOG and/or behavioral tests. Chemical analysis has been used successfully... 

Aberham A, Cicek SS, Schneider P, Stuppner H (2010) Analysis of sesquiterpene lactones, lignans, and flavonoids in wormwood (Artemisia absinthium 1.) using high-performance liquid chromatography (HPLC)-mass spectrometry, reversed phase HPLC, and HPLC-solid phase extraction-nuclear magnetic resonance. J Agric Food Chem 58 10817-10823... 

The purity control and the structure verification of compound collections from automated synthesis and combinatorial chemistry play an essential role in the success of medicinal chemistry programs. High performance liquid chromatography (HPLC), mass spectrometry (MS), and liquid chromatography-mass spectrometry (LC-MS) techniques are generally accepted as the most appropriate means of characterization (1,2). While these analytical methods are fast and easy to automate, they do not provide sufficient structural and quantitative data about the desired products. 

S)-(l-(3-(l,3-dimethyl-lH-pyrazol-5-yl)benzyl)pyrrolidin-2-yl)methanol To a 2-dram, round-bottom vial was added sequentially 3-formylphenyl MIDA boronate (0.4 M (THF suspension), 0.5 mL, 0.2 mmol), sodium triacetoxyborohydride (0.8 M (THF suspension), 0.5 mL, 0.4 mmol) and (5)-Prolinol (0.8 M (THF), 0.5 mL, 0.4 mmol), and the reactions were shaken at ambient temperature for 18 h. To the reaction vial was added a solution of aqueous K2CO3 (2 M, 1 mL, 2 mmol) and the reaction was then transferred to a 2-5 mL microwave vial containing 5-bromo-l,3-dimethyl-l//-pyrazole (0.4 mmol). To the vial was added a solution of PEPPSI-IPr in MeCN (0.1 M, 10 pmol), and the vials were irradiated in a microwave reactor at 150 °C for 20 min and analyzed by high-profile liquid chromatography (HPLC)-mass spectrometry (MS). To the microwave vial was added... 

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