High-performance liquid degradation products

Herbach, K.M., Stintzing, E.C., and Carle, R., Identification of heat-induced degradation products from purified betanin, phyUocactin and hylocerenin by high-performance liquid chromatography/electrospray ionization mass spectrometry, Rapid Commun. Mass Spectrom., 19, 2603, 2005 20, 1822, 2006. 

Zhang and Nunes [92] studied the structure and the generation mechanism of a novel degradation product formed by oxidatively induced coupling of miconazole nitrate with butylated hydroxy toluene in a topical ointment studied by high performance liquid chromatography-electrospray ionization mass spectrometry and organic synthesis. 

Tsuji and Goetz24 developed a quantitative high performance liquid chromatographic method for separating and measuring erythromycins A, B, and C, their epimers and degradation products. This method uses a /iBondapak Ci 8 reverse column with acetonitrile-methanol-O.2m ammonium acetate-water (45 10 10 25) as solvent. The pH and composition of the mobile phase may be adjusted to optimize resolution and elution volume. The authors utilized the procedure on USP reference standard and report a relative standard deviation of 0.64%. 

Backes, D.W., Aboleneen, H.I., and Simpson, J.I., Quantitation of vancomycin and its crystalline degradation product (CDP-1) in human serum by high performance liquid chromatography, 7. Pharm. Biomed. Anal., 16, 1281, 1998. 

KieBling, P., Scrlba, G. K. E., SiiB, E, Werner, G., Knoth, H., and Hartmann, M. (2004). Development and validation of a high-performance liquid chromatography assay and a capillary electrophoresis assay for the analysis of adenosine and the degradation product adenine in infusions.. Pharm. Biomed. Anal. 36(3), 535—539. 

Kirchoff, C., Bitar, Y., Ebel, S., and Holzgrabe, U. (2004). Analysis of atropine, its degradation products and related substances of natural origin by means of reversed phase high performance liquid chromatography./. Chromatogr. A 1046, 115-120. 

Kaune, A., Bruggemann, R., and Kettrup, A. High-performance liquid chromatographic measurement of the 1-octanol-water partition coefficients of s-triazine herbicides and some of their degradation products, J. Chromatogr. A, 805 (1-2) 119-126. 1998. 

Certain classes of lipids are susceptible to degradation under specific conditions. For example, all ester-linked fatty acids in triacylglycerols, phospholipids, and sterol esters are released by mild acid or alkaline treatment, and somewhat harsher hydrolysis conditions release amide-bound fatty acids from sphingolipids. Enzymes that specifically hydrolyze certain lipids are also useful in the determination of lipid structure. Phospholipases A, C, and D (Fig. 10-15) each split particular bonds in phospholipids and yield products with characteristic solubilities and chromatographic behaviors. Phospholipase C, for example, releases a water-soluble phosphoryl alcohol (such as phosphocholine from phosphatidylcholine) and a chloroform-soluble diacylglycerol, each of which can be characterized separately to determine the structure of the intact phospholipid. The combination of specific hydrolysis with characterization of the products by thin-layer, gas-liquid, or high-performance liquid chromatography often allows determination of a lipid structure. 

A Stitz, W Buchberger. Determination of sugar, sugar degradation products and alcohols by high-performance liquid chromatography with electrochemical detection. Fresenius J Anal Chem 339 55-57, 1991. 

S Motellier, IW Wainer. Direct stereochemical resolution of aspartame stereoisomers and their degradation products by high-performance liquid chromatography on a chiral crown ether based stationary phase. J Chromatogr 516 365-373, 1990. 

Y Ishi. Residue analysis of thiophanate methyl and its degradation products and metabolites by high performance liquid chromatography. J Pestic Sci 15 212-216, 1990. 

Wada, T., Nagasawa, E. and Hanaoka, S. (2006) Simultaneous determination of degradation products related to chemical warfare agents by high-performance liquid chromatography/mass spectrometry. Applied Organometallic Chemistry, 20(9), 573-79. 

Lamouroux, C., Aychet, N., Lelievre, A., Jankowski, C.K., Moulin, C. 2004. High-performance liquid chromatography with electrospray ionization mass spectrometry and diode array detection in the identification and quantification of the degradation products of calix[4]arene crown-6 under radiolysis. Rapid Commun. Mass Spectrom. 18 1493-1503. 

High-performance liquid chromatography has been used for the separation of Amadori products (2 ) and for the isolation of 3,8-dihydroxy-2-methylchromone from the products of xylose degradation at 100° (J3). In 1981 (4j we reported an HPLC separation scheme for the colored products of the xylose/glycine reaction. 

Terbuthylazine and degradation products Hot acetone, then cation exchange solid-phase cartridge High-performance liquid chromatograph with photodiode array detection [74,75]... 

Pietta P, Rava A, Biondi P. High-performance liquid chromatography of nifedipine, its metabolites and photochemical degradation products. J Chromatogr 1981 210 516-521. 

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