Capillary electrophoresis assay

Sabbah, S., and Scriba, G. K. E. (2001). Development and validation of a capillary electrophoresis assay for the determination of 3,4-diaminopyridine and 4-aminopyridine including related substances./. Chromatogr. A 907(1-2), 321-328. 

Busby WF, Ackermann JM, Crespi CL (1999) Effect of methanol, ethanol, dimethyl sulfoxide, and acetonitrile on in vitro activities of cDNA-expressed human cytochromes P-450. Drug Metab Dispos 27 246-249 Clohs L, Wong J (2002) Validation of a capillary electrophoresis assay for assessing the metabolic stability of verapamil in human liver microsomes. J Cap Elec Microchip Tech 7 113 Coughtrie MWH, Fisher MB (2003) The role of sulfotransferase and UDP-glucuronosyltransferases. In Lee JS, Obach RS, Fisher MB (eds) Drug Metabolizing Enzymes. Marcel Dekker, New York pp 541-575... 

Similarly, the sample preparation must be complementary to the instrumental method. Chen and Pollack (1) compared four sample preparation techniques for a capillary electrophoresis assay of a peptide in human plasma. Table 3 is a... 

In separate experiments, chickens, mice, or marmosets (nonhuman primates) inoculated with human adenovirus Ad-36 developed a syndrome of increased adipose tissue and paradoxically low levels of serum cholesterol and triglycerides (Dhurandhar et al., 1992, 2000, 2001). Food intake of the infected and the uninfected controls was not significantly different. Using a capillary electrophoresis assay (Kolesar et al., 2000), Ad-36 DNA was detected in the adipose tissue of the infected animals but not in the skeletal muscle. The amount of Ad-36 DNA present in the visceral fat correlated with the amount of total visceral fat in the chickens (r = 0.41,/ = 0.025). Sections of the brain and hypothalamus of Ad-36-inoculated animals did not show any overt histopathologic changes. 

O Brien, K. B. et al. A high-throughput on-line microdialysis-capillary electrophoresis assay for D-serine. Electrophoresis 24, 1227, 2003. 

Clohs, L. and Wong, J., Validation of a capillary electrophoresis assay for assessing the metabolic stability of verapamil in human liver microsomes, J. Capillary Electrophor, 1,113-117, 2002. 

Ruta J, Ravelet C, Baussane 1 et al (2008) Competitive affinity capillary electrophoresis assay based on a hybrid pre-incubation/on-capillary mixing format using an enantioselective aptamer as affinity ligand. J Sep Sci 31 2239-2243... 

The developed assay was successfully applied for the arsenite and arsenate determination in contaminated waters of the gold recovery plant and in snow covers of the industrial anthropogenic sources vicinities as well. The data produced are in a good agreement with the results of independent methods atomic absorptioin and atomic emission spectrometry and capillary electrophoresis. 

Enzyme-Linked Immunoadsorbent Assay (NRL) Multiplexed Sandwich Fluoroimmunoassay (LLNL) Capillary Electrophoresis (LLNL)... 

Another method which uses capillary electrophoresis with laser-induced fluorescence detection can also be employed to detect zearalenone (Maragos and Appell 2007). In order to analyse trace amounts of zearalenone in plants, a sensitive, quick and accurate method, the enzyme-linked immunosorbent assay (ELISA) was developed by Chen et al. 1989. 

Capillary electrochromatography-mass spectrometry (CE-MS), 4 641 Capillary electrodes, 14 27 Capillary electrophoresis (CE), 4 602-603, 631-633 6 385 9 751-752 antibody based columns with, 6 402 chiral additives, 6 77-79 applications, 4 641 basic principles, 4 606-609 detectors, 4 634-635 for DNA analysis, 4 636-637 flow profiles generated, 4 608 instrumentation, 4 633 as microfluidic assay technique,... 

With capillary electrophoresis (CE), another modern primarily analytically oriented separation methodology has recently found its way into routine and research laboratories of the pharmaceutical industries. As the most beneficial characteristics over HPLC separations the extremely high efficiency leading to enhanced peak capacities and often better detectability of minor impurities, complementary selectivity profiles to HPLC due to a different separation mechanism as well as the capability to perform separations faster than by HPLC are frequently encountered as the most prominent advantages. On the negative side, there have to be mentioned detection sensitivity limitations due to the short path length of on-capillary UV detection, less robust methods, and occasionally problems with run-to-run repeatability. Nevertheless, CE assays have now been adopted by industrial labs as well and this holds in particular for enantiomer separations of chiral pharmaceuticals. While native cyclodextrins and their derivatives, respectively, are commonly employed as chiral additives to the BGEs to create mobility differences for the distinct enantiomers in the electric field, it could be demonstrated that cinchona alkaloids [128-130] and in particular their derivatives are applicable selectors for CE enantiomer separation of chiral acids [19,66,119,131-136]. 

Rocheleau, M.-J. Generic capillary electrophoresis conditions for chiral assay in early pharmaceutical development. Electrophoresis 2005, 26, 2320-2329. 

Capillary electrophoresis (CE) has become a valuable technique in the analytical toolbox for pharmaceutical analysts. CE methods have been successfully applied for identification, assay, purity determination, and chiral separation. ICH guidelines should be followed in meeting regulatory approval if CE methods are used in a registration dossier. Here, the validation parameters required for different analytical procedures are described and a comprehensive overview of CE validation studies presented in literature is given. 

During the last decade capillary electrophoresis (CE) has become a mature separation technique for pharmaceutical analysis. Numerous validated methods from pharmaceutical R D lahoratories and academia have been reported in literature, including identity confirmation, main component assay, purity determination, enantiomeric separation, and stoichiometry determination. In addition, CE is frequently applied as an orthogonal technique during the development of stability indicating liquid chromatography methods. As a result CE... 

Liu, L., Osborne, L. M., and Nussbaum, M. A. (1996). Development and validation of a combined potency assay and enantiomeric purity method for a chiral pharmaceutical compound using capillary electrophoresis. J. Chromatogr. A 745(1—2), 45 —52. 

Perez-Rulz, T., Martinez-Lozano, C., Sanz, A., and Bravo, E. (2004). Development and validation of a quantitative assay for raloxifene by capillary electrophoresis. /. Pharm. Biomed. Anal. 34(5), 891-897. 

Altria, K. D., Frake, P., Gill, 1., Hadgett, T., Kelly, M. A., and Rudd, D. R. (1995). Validated capillary electrophoresis method for the assay of a range of basic drugs.. Pharm. Biomed. Anal. 13,... 

Owens, P. K., Wikstrdm, H., Nagard, S., and Karlsson, L. (2002). Development and validation of a capillary electrophoresis method for ximelagatran assay and related substance determination in drug substance and tablets.. Pharm. Biomed. Anal. 27, 587-598. 

Page, J. S., Rubakhin, S. S., and Sweedler, J. V. (2000). Direct cellular assays using off-line capillary electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Analyst 125, 555-562. 

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