High performance liquid chromatography diode array detectors

Yang, S.Y., Pan, G.M., Meng, G.F., Zhang, D.M. (2001). Study of diphacinone in biological samples by high performance liquid chromatography/diode array detector. Se Pu 19(3) 245-7. (In Chinese)... [Pg.225]

GC—gas chromatography HCA—hierarchical cluster analysis HELP—heuristic evolving latent projections HHM—horse heart myoglobin HPLC—high performance liquid chromatography HPLC-DAD—high performance liquid chromatography-diode array detector... [Pg.463]

Erdogan S, Erdemoglu S (2011) Evaluation of polyphenol contents in differently processed apricots using accelerated solvent extraction followed by high-performance liquid chromatography diode array detector, hit J Food Sci Nutr 62(7) 729-739... [Pg.2039]

In the last twenty years, many of the developed and validated high performance liquid chromatography methods with conventional diode array or fluorescence detectors (DAD, FLD) were improved and substituted by new hyphenation with mass spectrometric instrumentation and/or NMR, especially for the analyses of raw materials derived from Natural sources. The main goal of this coupling is achieved by improvement of selectivity and sensitivity of new instrumental configurations [7], Furthermore, with these configurations it is possible to obtain, in only one analysis, the complete chemical structure elucidation, identification and quantification of targeted compounds. [Pg.49]

High-Performance Liquid Chromatography. A Varian 5060 delivery system was used for this work with detection by UV absorption. Either a Varian UV-50 variable wavelength detector or a Hewlett Packard 1040A scanning diode array detector was used. All HPLC columns were packed in our laboratory (10) with 5-/um particle size Spherisorb-ODS, Spherisorb-CN (Phase Separations), or 8-pm particle size Zorbax-CN (Dupont Ltd). HPLC columns (20 or 25 cm X 4.6 mm i.d.) were coupled via short lengths of stainless steel capillary tubing (5 cm X 0.25 mm i.d.). Separation conditions were as follows ... [Pg.641]

J,m poly(tetrafluoroethylene) (PTFE) syringe-tip filters High-performance liquid chromatography (HPLC) system equipped with Quaternary pump Diode array detector Vacuum degasser... [Pg.1251]

Notes LOD, limit of detection MeOH, methanol EtOH, ethanol ACN, acetonitrile EtAC, ethyl acetate SPE, solid phase extraction HLB (hydrophilic lipophilic balanced) TFA, trifluoroacetic acid GC, gas chromatography TMS, trimethylsilyl MS, mass spectrometry HPLC, high-performance liquid chromatography DAD, diode array detector NMR, nuclear magnetic resonance ESI, electrospray ionization APCI, atmospheric pressure chemical ionization CE, capillary electrophoresis ECD, electrochemical detector CD, conductivity detector TLC, thin layer chromatography PDA, photodiode array detector. [Pg.65]

R. A. de Zeeuw, J. P. Franke, and M. Bogusz, High performance liquid chromatography with a multichannel diode-array spectro-photometric detector in systematic toxicological analysis. In Analytical Methods in Forensic Chemistry (M. H. Ho, ed.), Ellis Horwood, New York, 1990, p. 212. [Pg.222]

G. Cavina, L. Valvo, B. Gallinella, R. Porra, and A. L. Savella, The identification of related substances in 9a-fluoroprednisolone-21 acetate by means of high-performance liquid chromatography with diode array detector and mass spectrometry, J. Pharm. Biomed. Anal., 79 437(1992). [Pg.354]

Zavitsanos, P., and Goetz, H. (1991). The practical application of diode array UV-visible detectors to high-performance liquid chromatography analysis of peptides and proteins. In High-Performance Liquid Chromatography of Peptides and Proteins Separation, Analysis,... [Pg.66]

Quantitahve analysis of reaction mixtures was performed by high-performance liquid chromatography (HPLC) and a diode array ultraviolet detector, as reported elsewhere [5, 6, 17]. [Pg.125]

Reversed-phase high-performance liquid chromatography (RP-HPLC) is the usual method of choice for the separation of anthocyanins combined with an ultraviolet-visible (UV-Vis) or diode-array detector (DAD)(Hebrero et al., 1988 Hong et ah, 1990). With reversed-phase columns the elution pattern of anthocyanins is mainly dependent on the partition coefficients between the mobile phase and the Cjg stationary phase, and on the polarity of the analytes. The mobile phase consists normally of an aqueous solvent (water/carboxylic acid) and an organic solvent (methanol or acetonitrile/carboxylic acid). Typically the amount of carboxylic acid has been up to 10%, but with the addition of a mass spectrometer as a detector, the amount of acid has been decreased to as low as 1 % with a shift from trifluoroacetic acid to formic acid to prevent quenching of the ionization process that may occur with trifluoroacetic acid. The acidic media allows for the complete displacement of the equilibrium to the fiavylium cation, resulting in better resolution and a characteristic absorbance between 515 and 540 nm. HPLC separation methods, combined with electrochemical or DAD, are effective tools for anthocyanin analysis. The weakness of these detection methods is a lack of structural information and some nonspecificity leading to misattribution of peaks, particularly with electrochemical... [Pg.165]

Among the three advantages that diode array detector provides to the high-performance liquid chromatography (HPLC) analyst, peak identification is the one that allows the identification of unknown constituents in a sample whose matrix is complex. [Pg.1114]

High-performance liquid chromatography is performed using a Hewlett-Packard 1090 chromatograph equipped with a ternary-solvent delivery system, an autoinjector with a 0 -20- u.L injection loop, an oven compartment, and a diode-array UV detector. An ELS detector (Alltech Associates, Deerfield, IL) is connected in series to the UV detector. Hexane, 2-propanol, and water were used for the analysis of nonionic surfactants. Water and tetrahydrofuran (THF) are used for the analysis of anionic surfactants. No preliminary sample preparation is used other than dilution. The nonionic surfactants are diluted 1 40 (v/v) with hexane. The anionic surfactants (alkyl ether sulfates and synthetic and petroleum sulfonates) are diluted 1 20 (v/v) with water-THF (50 50). The calcium sulfonate surfactants were diluted 1 20 (v/v) with a THF-38% hydrochloric acid solution of pH 1. Hydrochloric add is required to prevent salt precipitation by converting any excess water-insoluble caldum carbonate into water-soluble calcium chloride. All diluted samples are... [Pg.1559]