High performance liquid chromatography detection

Mitchell TW, Savage JC, Gould DH. 1993. High-performance liquid-chromatography detection of sulfide in tissues from sulfide-treated mice. J Appl Toxicol 13 389-394. 

Determination of mandelic acid in urine has been recommended as a biomaiker of exposure to ethylbenzene. Several methods can be used to determine mandelic acid in urine samples. These include derivatization of the acid and GC analysis (detection limit, 1.0 mg/L) isotachophoresis (detection limit, 0.04 mmol/L) and high-performance liquid chromatography (detection limit, 0.01 mmol/L) (WHO, 1996a). 

Imazupyr Methanol None High-performance liquid chromatography, detection at 250 nm [73]... 

Matthees, D.P. and Purdy, W.C., Naphthyldiazomethane as a derivatizing agent for the high-performance liquid chromatography detection of bile acids, Anal. Chim. Acta, 109, 161, 1979. 

P. E. Nelson, S. L. Nolan, and K. R. Bedford, High-performance liquid chromatography detection of morphine by fluorescence after post-column derivatization, J. Chromatogr., 234 407 (1982). 

H. Gunasingham, B. T. Tay, and K. P. Ang, Automated Mercury Film Electrode for Flow Injection Analysis and High-Performance Liquid Chromatography Detection. Anal. Chem., 58 (1986) 1578. 

Jeurissen, S.M., F.W. Claassen, J. Havlik, et al. 2007. Development of an on-line high performance liquid chromatography detection system for human cytochrome P450 1A2 inhibitors in extracts of natural products. /. Chromatogr. A 1141(l) 81-89. 

It is useful for the cell holder position to be adjustable so that the crucial portion of the sample that is illuminated by the light source and observed by the detector is offset from the center of the cuvette this helps to minimize inner filter effects. Some commercial instruments provide this facility. A great range of flow cells for use in flow analysis and high-performance liquid chromatography detection is available these generally have very short optical pathlengths, so inner filter effects are much less likely. 

Figure 5.3 Analysis of 100 ml of (a) surface water and (b) drinking water sample spiked with 0.1 pig/ml of microcystins, using column-switching HPLC 1, microcystin-RR 2, microcystin-YR 3, microcystin-LR. Reprinted from Journal of Chromatography A, 848, H. S. Lee et al, On-line trace enrichment for the simultaneous determination of microcystins in aqueous samples using high performance liquid chromatography with diode-array detection , pp 179-184, copyright 1999, with permission from Elsevier Science.

A. J. Szuna and R. W. Blain, Determination of a new antibacterial agent (Ro 23-9424) by multidimensional high-performance liquid chromatography with ulti aviolet detection and direct plasma injection , 7. Chromatogr. 620 211-216 (1993). 

For off-bead analysis, coupling between chromatographic separation and mass spectrometric detection has proven especially powerful. The combination between high performance liquid chromatography (HPLC) and electrospray ionisation mass spectrometry has the advantage that purity of product mixtures can be coupled on-line with the product identification. 

Figure 3 depicts profiles of Total PAH fluxes vs. time (36). The following polycyclic hydrocarbons have been determined by high performance liquid chromatography, variable wavelength absorption detection Naphthalene, acenaphthylene, 7,12-dimethylbenzanthracene, 2-methylnaphtalene, fluorene, acenaphtene, phenanthrene, 2,3-dimethylnaphtalene, anthracene, fluoranthene, 1-methylphenanthrene, pyrene, 2,3-benzofluorene, triphenylene, benz(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, perylene, benzo(e)pyrene, 1,2,3,4-dibenzanthracene, benzo(a)pyrene, and 1,2,5,6-dibenzanthracene. 

In the ED setting, the diagnosis of ketamine intoxication is a clinical one. Ketamine is not routinely detected by urine toxicology tests, although it can be detected with high-performance liquid chromatography (Koesters et al. 2002). As with MDMA, the initial assessment for ketamine intoxication includes the use of routine laboratory tests to detect electrolyte abnormalities and to evaluate renal and hepatic functioning (Koesters et al. 2002). 

Clark GJ, Goodin RR, Smiley JW. 1985. Comparison of ultraviolet and reductive amperometric detection for determination of ethyl and methyl parathion in green vegetables and surface water using high-performance liquid chromatography. Anal Chem 57 2223-2228. 

The use of high performance liquid chromatography (HPLC) for the study of paralytic shellfish poisoning (PSP) has facilitated a greater understanding of the biochemistry and chemistry of the toxins involved. HPLC enables the determination of the type and quantity of the PSP toxins present in biological samples. An overview of the HPLC method is presented that outlines the conditions for both separation and detection of the PSP toxins. Examples of the use of the HPLC method in toxin research are reviewed, including its use in the determination of the enzymatic conversion of the toxins and studies on the movement of the toxins up the marine food chain. 

DE PASCUAL-TERESA S, TREUTTER D, RIVAS-GONZALO J 0 and SATOS-BUELGA C (1998) Analysis of flavonols in beverages by high-performance liquid chromatography with chemical reaction detection , J Agric Food Chem, 46, 4209-13. 

KAYALI-SAYADI M N, RUBIO-BARROSO S, CUESTA-JIMENEZ M P and PALO-DIEZ L M (1998) Rapid determination of polycyclic aromatic hydrocarbons in tea infusion samples by high-performance liquid chromatography and fluorimetric detection based on solid-phase extraction , 123, 2145-8. 

GUO c, CAO G, SOFIC E and PRIOR R L (1997) High-performance liquid Chromatography coupled with coulometric array detection of electroactive components in fruits and vegetables Relationship to oxygen radical absorbance capacity, J Agric Food Chem, 45, 1787-96. 

JUSTESEN u, KNUTHSEN p and LETH T (1998) Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by high-performance liquid chromatography with photo-diode array and mass specfrometric detection, /C/u matogr A, 799, 101-10. 

Paganga, G. et al.. The polyphenolic content of fruit and vegetables and their antioxidant activities what does a serving constitute Free Radical Res., 30, 153, 1999. Maatta, K.R. et al.. High-performance liquid chromatography (HPLC) analysis of phenolic compounds in berries with diode array and electrospray ionization mass spectrometric (MS) detection Rihes species, J. Agric. Food Chem., 51, 6736, 2003. 

Kammerer, D., Carle, R., and Schieber, A., Detection of peonidin and pelargonidin glycosides in black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) by high-performance liquid chromatography/electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom., 17, 2407, 2003. 

Gandla-Herrero, R, Garcla-Carmona, R, and Escribano, J., A novel method using high-performance liquid chromatography with fluorescence detection for the determination of betaxanthins, J. Chromatogr. A, 1078, 83, 2005. 

Saito, K., A new enzymatic method for extraction of precarthamin from dyer s saffron (Carthamus tinctorius) florets, Z. Lebensmitt. Untersuch. Forsch., 197, 34, 1993. Cserhati, T. et ah. Separation and quantitation of colour pigments of chili powder (Capsicum frutescens) by high-performance liquid chromatography-diode array detection, J. Chromatogr. A, 896, 69, 2000. 

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