High performance liquid chromatography UV detection

For purity and/or impurity analysis using high-performance liquid chromatography, UV detection is still the method of choice for the reasons discussed earlier. In brief,... 

Rapala, I, Erkomaa, K., Kukkonen, I, Sivonen, K., and Lahti K. 2002. Detection of microcystins with protein phosphatase inhibition assay, high performance liquid chromatography-UV detection and enzymelinked immunosorbent assay. Comparison of methods. Analytica ChimicaActa (466) 213- 231. 

Cai, Y., Jiang, G., and Liu, J., Solid-phase microextraction coupled with high performance liquid chromatography-UV detection for the determination of di-n-propyl-phthalate, di-iso-butyl-phthalate, and di-cyclohexyl-phthalate in environmental water samples. Anal Lett, 36, 389-404, 2003. 

Yamada, K. Sakamoto, Y. Satoh, T. A simple method for the measurement of pineal melatonin with a high performance liquid chromatography-UV detection, Res.Commun.Chem.Pathol.Pharmacol., 1984, 46, 283-287. 

These authors noted the potential for the assay to underestimate the concentration of TSR due to decreased binding of metabolites relative to parent spinosad. However, the major residue found was parent spinosad, so underestimation of residues is not likely to be problematic. Overall, this method was validated in 34 matrices and showed excellent agreement with results obtained with a high-performance liquid chromatography/ultraviolet detection (HPLC/UV) method. ... 

P.A. Tarantilis, G. Tsoupras and M. Polissiou, Determination of saffron (Crocus sativus L.) components in crude plant extract using high performance liquid chromatography UV visible photodiode array detection mass spectrometry, J. Chromatogr. A, 699, 107 118 (1995). 

In modern times, most analyses are performed on an analytical instrument for, e.g., gas chromatography (GC), high-performance liquid chromatography (HPLC), ultra-violet/visible (UV) or infrared (IR) spectrophotometry, atomic absorption spectrometry, inductively coupled plasma mass spectrometry (ICP-MS), mass spectrometry. Each of these instruments has a limitation on the amount of an analyte that they can detect. This limitation can be expressed as the IDL, which may be defined as the smallest amount of an analyte that can be reliably detected or differentiated from the background on an instrument. 

High-performance liquid chromatography (HPLC) with a micellar mobile phase or with a selective pre-column or reaction detection system has also been used to determine alkylenebis(dithiocarbamaes). ° Zineb and mancozeb residues in feed were determined by ion-pair HPLC with ultraviolet (UV) detection at 272 nm. These compounds were converted to water-soluble sodium salts with ethylenediaminetetra-acetic acid (EDTA) and sodium hydroxide. The extracts were ion-pair methylated with tetrabuthylammonium hydrogensulfate (ion-pair reagent) in a chloroform-hexane solvent mixture at pH 6.5-8.S. The use of an electrochemical detector has also been reported. ... 

Residue analytical methods for neonicotinoids in crops, soil and water samples have been developed. The basic principle of these methods consists of the following steps extraction of the crop and/or soil samples with acetone or the other organic solvent, cleanup by liquid-liquid partition or column chromatography, and quantitative analysis by high-performance liquid chromatography with ultraviolet detection (HPLC/UV). Simple column cleanup procedures are used to improve the accuracy and sensitivity of these methods. 

Engel, R. (1988) Determination of Thiophanate-methyl by High Performance Liquid Chromatography and UV Detection, Report BT-41, Medical Biological Laboratory TNO, Department of Occupational Toxicology, Rijswijk, The Netherlands (in Dutch). 

The use of direct UV spectrophotometry to measure sample concentrations in pharmaceutical research is uncommon, presumably due to the prevalence and attractiveness of high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS) methods. Consequently, most researchers are unfamiliar with the value of UV detection, mainly that it is generally much faster than other methods - a very important asset in high-throughput screening. 

B. Szostek, J. Orska Gawrys, I. Surowiec and M. Trojanowicz, Investigation of natural dyes occurring in historical Coptic textiles hy high performance liquid chromatography with UV vis and mass spectrometric detection, Journal of Chromatography, A, 1012, 179 192 (2003). 

Figure 7.5 Schematic diagram of a high performance liquid chromatography (HPLC) system. The solvent(s) are pumped through the system, and the sample injected just before the column where separation occurs. Detection is often by UV/visible spectrophotometry at a fixed wavelength.

Chromatographic systems have one thing in common most depend on spectro-photometric detection devices, i.e., ultraviolet (UV), visible, fluorescent, and midrange infrared (MIR) spectrometers. High-performance liquid chromatography (HPLC) has been used to, in essence, purify (separate) the constituents from the matrix, then introduce them to a spectrometer for identification or quantification. One reason that spectrometers were not placed in a production setting... 

Duverneuil C, Grandmaison GL, Mazancourt P, Alvarez JC. 2003. A high-performance liquid chromatography method with photodiode-array UV detection for therapeutic drug monitoring of the nontricyclic antidepressant drugs. Ther Drug Mon 25(5) 565-573. 

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