Herpes Simplex virus type 1 thymidine kinase

The Case Study Herpes Simplex Virus Type 1 Thymidine Kinase Substrates and Inhibitors... 

Otero, G.C. and Hope, T.J. (1998) Splicing-independent expression of the herpes simplex virus type 1 thymidine kinase gene is mediated by three cis-acting RNA sub-elements. J. Virol, 72, 9889-9896. 

Black, M. E., Newcomb, T. G., Wilson, H. M., and Loeb, L. A. (1996). Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proc. Natl. Acad. Sci. USA, 93, 3525-3529. 

Rainov NG. A phase III clinical evaluation of herpes simplex virus type 1 thymidine kinase and ganciclovir gene therapy as an adjuvant to surgical resection and radiation in... 

Figure 13.5 Schematic representation for imaging Herpes Simplex virus type 1-thymidine kinase...

Haubner R, Avril N, Hantzopoulos PA, Gansbacher B, Schwaiger M. In vivo imaging of herpes simplex virus type 1 thymidine kinase gene expression early kinetics of radiolabelled FIAU. Eur J Nucl Med 2000 27 283-291. 

Gambhir SS, Bauer E, Black ME, Liang Q, Kokoris MS, Barrio JR, Iyer M, Namavari M, Phelps ME, Herschman HR et al. A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography. Proc Natl Acad Sci USA 2000 97(6) 2785-2790. 

Herpes simplex virus type 1 (thymidine/thymidylate multifunctional kinase [5]) [5]... 

Chen, M.C. Walker, J. Prusoff, W.H. Kinetic studies of herpes simplex virus type 1-encoded thymidine and thymidylate kinase, a multifunctional enzyme. J. Biol. Chem., 254, 10747-10753 (1979)... 

Penciclovir [pen SIK lo veer] is an acyclic guanosine nucleoside derivative that is active against herpes simplex virus Types I and II, and against varicella-zoster virus. Penciclovir is only administered topically (Figure 37.8). Penciclovir is monophosphorylated by viral thymidine kinase, and cellular enzymes form the nucleoside triphosphate, which inhibits herpes DNA polymerase. Penciclovir triphosphate has an intracellular half-life 20 to 30 times longer than does acyclovir triphosphate (see p. 365). Penciclovir is negligibly absorbed from topical application, and is well tolerated. Both healing and pain are shortened approximately one-half day in duration, compared to placebo-treated subjects. 

Aciclovir is active against Herpes simplex virus type 1 (HSV-1), HSV-2, Varicella zoster virus (VZV), Herpesvirus simiae, and to a lesser degree Epstein-Barr virus (EBV). Resistant strains of HSV can arise owing to the emergence of thymidine kinase-deficient mutants. Other forms of resistance patterns are less common (2,3). 

Wei C M, Gibson M, Spear P G, et al. (1981). Construction and isolation of a transmissible retrovirus containing the src gene of Harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. J. Virol. 39 935-944. 

Iqbal, J., Scapozza, L., Eolkers, G, and Muller CE., Development and validation of a capillary electrophoresis method for the characterization of herpes simplex virus type 1 (HSV-1) thymidine kinase substrates and inhibitors. J. Chromatogr. B-Anal. Technol. Biomed. Life Sci., 846, 281-290, 2007. Wang, W, Tang, J., Wang, S., Zhou, L., and Hu, Z., Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection, J. Chromatogr. A, 2007. Vol 1148(1) 108-114. 

T. Osaki, Y. Tanio, I. Tachibana, S. Hosoe, T. Kumagai, I. Kawase, S. Oikawa, and T. Kishimoto, Gene therapy for carcinoembryonic antigen-producing human lung cancer cells by cell type-specific expression of herpes simplex virus thymidine kinase gene, Cancer Res. 54 5258 (1994). 

Viral herpes simplex type 1 thymidine kinase (HSVl TK) is a key enzyme in the metabolism of the herpes simplex virus. Its physiological role is to salvage thymidine into the DNA metabolism by converting it to thymidine monophosphate ... 

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