Herpes simplex virus, detection

Fig. 10 Herpes simplex virus detection. Validation for specific detection of herpes simplex virus using REVS a 500000 HSV (gD+) virions incubated on a surface coated with an HSV receptor (anti-gD monoclonal antibody), b the same number of HSV virions on a surface with a receptor to which HSV does not bind (control bovine IgG), c genetically modified HSV (gD ) on HSV receptor surface, d HSV on a soluble gD-blocked HSV receptor surface, e HSV in blocking solution (anti-gD monoclonal antibody) on HSV receptor surface. The traces have been displaced from zero on the y-axis for clarity...

Human embryo lung fibroblasts infected with a reference laboratory strain of herpes simplex virus (HS V) type 2 were used to detect antibody to HSV type 2 in serum samples. After treatment of cells with serial dilutions of sera, HRP-labeled immunoglobulins to human IgG (class G immunoglobulins) were added and detected with CL substrate [36], In both cases a sharp detection of the specific antibodies was achieved with chemiluminescent assays, which proved more sensitive than the colorimetric immunoperoxidase assays. 

M.J. Espy, J.R. Uhl, L.M. Sloan, J.E. Rosenblatt, F.R. Cockerill and T.F. Smith, Detection of Vaccinia virus, Herpes Simplex virus, Varicella-Zoster... 

Hukkanen, V. Rehn, T. Kajander, R. Sjoroos, M. Waris, M. Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid. J. Clin. Microbiol. 2000, 33(9), 3214-3218. 

Bernstein, D. I., Garraty, E., Lovett, M. A., and Bryson, Y. J. (1985) Comparison of western blot analysis to microneutralization for the detection of type-specific antibodies to herpes simplex virus antibodies. J. Med. Virol. 15, 223-230. 

Herpes Simplex Virus Type 1 (HSV-I). The RIA was compared to two standard serologic tests for detecting antibody to HSV-1 the IHA test and the CF test. Table V shows the results obtained with sera from three different sources. The overall agreement in distinguishing between positive and negative sera was 98%. 

The Precision of the Magnetic Radioimmunoassay for Detecting Antibody to Herpes Simplex Virus Type I (HSV-I)... 

Several methods are available for rapid detection of VZV in clinical specimens. Although a Tzanck smear is not diagnostic of VZV, most local hospitals with a pathology laboratory can perform the test easily and quickly. A positive Tzanck smear confirms an alphaherpes virus infection, either VZV or HSV (Herpes Simplex Virus). 

Simko JP, Caliendo AM, Hogle K, Versalovic J. Differences in laboratory findings for cerebrospinal fluid specimens obtained from patients with meningitis or encephalitis due to herpes simplex virus (HSV) documented by detection of HSV DNA. 

Viral agents may be detected by direct observation of inoculated culture cells for cytopathic effects or by detection of antigens after incubation by immunofluorescent methods. The culture method is most useful for organisms such as cytomegalovirus (CMV) or herpes simplex virus because these viral agents are rapidly propagated in culture cells, making them easily detected. ... 

Cohen PR. Tests for detecting herpes simplex virus and varicella-zoster virus infections. Dermatol Clin. 1994 12 51-68. 

Wang JY, Montone KT. A rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe a comparison with immunohistochemical methods for HSV detection. J Clin Lab Anal. 1994 8 105-115. 

Nicoll JAR, Love S, Burton PA, et al. Autopsy findings in two cases of neonatal herpes simplex virus infection detection of virus by immunohistochemistry, in situ hybridization and the polymerase chain reaction. Histopathology. 1994 24 257-264. 

Gentilomi G, Musiani M, Zerbini M, et al. Double in situ hybridization for detection of herpes simplex virus and cytomegalovirus DNA using non-radioactive probes. J Histochem Cytochem. 1992 40 421-425. 

Other applications of this type of detection include Western blotting procedures for the identification and visualization of protein antigens (LI, L8, L9). One such recent assay for herpes simplex virus Type 2 showed that enhanced chemiluminescence is 500 times more sensitive than a colorimetric method (Dl). Antibodies to several of the HIV viral proteins have also been detected in chemiluminescent Western blots (SI6). 

B22. Bronstein, I., and Voyta, J. C., Chemiluminescence detection of herpes simplex virus I DNA in blot and in-situ hybridization assays. Clin. Chem. (Winston-Salem, N.C.) 35, 1856-1857... 

P15. Pronovost, A. D., Baumgarten, A., and Hsiung, G. D., Sensitive chemiluminescent enzyme-linked immunosorbent assay for quantification of human immunoglobulin G and detection of Herpes Simplex virus. J. Clin. Microbiol. 13, 97-101 (1981). 

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