In situ hybridization method

Childs, C.V., Lloyd, J.M., Unabia, C., Gharib, S.D., Wierman, M.E., and Chin, W.W. (1987) Detection of luteinizing hormone b messenger ribonucleic acid (RNA) in individual gonadotropes after castration Use of a new in situ hybridization method with a photobiotinylated complementary RNA probe. Mol. Endocrinol. 1, 926-932. 

Hopman, A.H.N., Wiegant, J., Tesser, G.I., and Van Duijn, R (1986) A nonradioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten hgands. Nucleic Acids Res. 14, 6471-6488. 

Abstract Quantitative receptor autoradiography methods have heen widely used over the last three decades to study the distribution and physiological role of a receptor in various tissues. This review provides an overview of in vivo and in vitro receptor autoradiography methods and their advantages as well as disadvantages in the study of receptors in the central nervous system. Comparison with immuno-histochemical and in situ hybridization methods is also highlighted in relation to the study of a given receptor in the nervous sytem. 

Tongiorgi E, Righi M, Cattaneo A. 1998. A non-radioactive in situ hybridization method that does not require RNAse-free conditions. J Neurosci Meth 85 129-139. 

Lawrence, J. B. and Singer, R. H. (1985) Quantitative analysis of in situ hybridization methods for the detection of actin gene expression. Nucleic Acids Res. 13, 1777-1799. 

Bright-Field In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples... 

Bright-field In Situ Hybridization Methods to Discover Gene. 

Nielsen BS (2012) MicroRNA in situ hybridization. Methods Mol Biol 822 67-84... 

K. Murakami, K. Yamada, K. Deguchi, T. Shimizu and S. Ando, Preparation of soluble polyimide/MgO nanohybrid films by in situ hybridization method and evaluation of their thermal conductivity. J. Photopolym. Sci. Technol, 2010, 23,501-506. 

Jowett, T. Tissue In situ Hybridization Methods in Animal Development John Wiley Sons, Inc. New York, 1997, p. 121. 

Radiation Personnel Accident. Radiation exposure in accidents of personnel in nuclear facilities and non-destructive detection have been evaluated with ESR dosimetry. For example, the amputated medium fibre bone of a worker in Peru, who tried to solve the problem by directly touching the source of 60Co unit for a few seconds, was measured four years later.The dose was measured by 6.4 + 0.5 Gy, while 20 + 3 Gy was obtained from fluorescence in situ hybridization method. The result would suggest diagenic decay of the signal due to the metabolism of bone.96 Thus, ESR gives the retrospective dose for radiation accidents. 

Sassoon, D and Rosenthal, N. (1993) Detection of messenger RNA by in situ hybridization Methods Enzymol 225, 384—404... 

Sohier, D. and Lonvaud-Funel, A. 1998. Rapid and sensitive in situ hybridization method for detecting and identifying lactic acid bacteria in wine. Food Microbiol. 15, 391-397. 

Brockman SR, Paternoster SR Ketterling RR Dewald GW. New highly sensitive fluorescence in situ hybridization method to detect PML/RARA fusion in acute promyelocytic leukemia. Cancer Genet Cytogenet 2003 145 144-151. 

Esclapez M, Tillakaratne NJK, Tobin AJ, Houser CR (1993) Comparative localization of mRNAs encoding two forms of glutamic acid decarboxylase with nonradioactive in situ hybridization methods. J. Comp. Neurol, 331, 339-362. 

Wang JY, Montone KT. A rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe a comparison with immunohistochemical methods for HSV detection. J Clin Lab Anal. 1994 8 105-115. 

As stated, the t(12 22)(ql3]2) translocation (yielding a hybrid EWS/ATF-1 gene) is characteristic of CCS. tp Its presence can be evaluated by in situ hybridization methods as well as PCR-based technologies, providing a useful diagnostic adjunct to immunohistology. 

Park K, Kim J, Lim S, et al. Comparing fluorescence in situ hybridization and chromogenic in situ hybridization methods to determine the HER2/neu status in primary breast carcinoma using tissue microarray. Mod Pathol 2003, 16, 937-943. 

DNLM 1. Neurotransmitters—physiology. 2. Histochemistry— methods. 3. In Situ Hybridization— methods. 4. Chromatography, Liquid— methods, 5. Spectrum Analysis, Mass— methods. W1 ME 9616J V. 72 1997/QV 126 N4939 1997]... 

Until recently, the application of direct fluorescent labeling in situ hybridization methods was limited, perhaps because the low sensitivity was overemphasized and commercial labeling systems were not available (complex chemistry was required to label the probe or nucleotides). The combination of enzymic labeling techniques and commercial fluorochromized nucleotides, with a better understanding... 

Hofler, H, Childers, H, Montminy, M. R., Lechan, R M, Goodman, R H., and Wolfe, H J. (1986) In situ hybridization methods for the detection of somatostatin mRNA in tissue sections using antisense RNA paubes Histochem. J 18, 597-604. 

Tautz, D. and Pfeile, C. (1989) A non-radioactive in situ hybridization method for the localization of specific RNA s in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98,81-85... 

Ahneida, C., Azevedo, N. R, Fernandes, R. M., Keevil, C. W., Vieira, M. J. (2010). Fluorescence in situ hybridization method using a peptide nucleic acid probe for identification of Salmonella spp. in a broad spectrum of samples. Applied and Environmental Microbiology, 76(13), 4476-4485. http //dx.doi.org/10.1128/AEM.01678-09 PMCID PMC2897454. 

We use a modified in-situ hybridization method to increase the subsequent performance of the antibody ... 

Tautz, D. and Pfeifle, C. (1989) A non-radioactive in situ hybridization method for the localization of specific RNAs in Drosophila embryos reveals translational control of the segmentation gene hunchback. Chromosoma 98,81-85. Hemmati-Brivanlou, A., Frank, D., Bolce, M. B., Sive, H. L., and Harland, R. M. (1990) Localization of specific mRNAs in Xenopus embryos by whole mount in situ hybridization. Development 110,325-330. 

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