Hematoxylin and eosin staining

Other zinc solutions, free of formaldehyde, have been proposed.29 31 All of these simple buffered salt solutions preserve immunoreactivity well and are suitable for DNA, RNA, and proteomics research. Judging by published photomicrographs of hematoxylin and eosin-stained specimens, cytological detail is inferior to that achieved with standard formalin. Nuclei are condensed to the point where many lack chromatin patterns.3132 Such zinc salt solutions may be good for specialized purposes but are best used as special fixatives. To get good structural detail as well, specimens should be split so that a portion can... 

Figure 21.4 (a) Hematoxylin and eosin staining of rat brain coronal section, and... 

Because myoepithelial cells are not always readily identifiable on routine hematoxylin- and eosin-stained sections, many immunohistochemical methods have been used to highlight an intact myoepithelial cells layer. Recent studies have reported CD10 and smooth muscle myosin heavy chain (SMMHC) expression in... 

In another liver foci study using Sprague-Dawley (Crl CD) rats, 1,2-dibromoethane in corn oil given by gavage was used as an initiator. Two dose regimens were used 75 mg/kg 1,2-dibromoethane at 0 and 24 hours or corn oil at 0 hours and 75 mg/kg 1,2-dibromoethane at 24 hours. Partial hepatectomies and phenobarbital in drinking water also were part of the protocol. With this system, at 16 months, 1,2-dibromoethane-exposed rats had increased numbers of foci of hepatic cellular alteration. Rats that received the two doses of 1,2-dibromoethane had increased numbers of nodules on hematoxylin and eosin-stained sections as well as increased number and size of GGT positive foci (Moslen 1984). These results indicate that 1,2-dibromoethane can act as an initiator. 

The presence of necrotic, inflammatory, and/or nontumorigenic tissue should be determined by a pathologist by reviewing the hematoxylin- and eosin-stained 5-pm section prepared in step 1. 

FIGURE 18.5 Light photomicrographs of microneedle-treated human skin stained for (3-galactosidase expression, (a) En face stereomicroscopy of dry-etch microneedle-treated skin with nuclear fast red counterstaining (b) en face stereomicroscopy of wet-etch microneedle-treated skin (c) hematoxylin and eosin stained 12 xm cryosection of dry-etch microneedle-treated skin. Bar =100 xm (d) hematoxylin and eosin stained 12 xm cryosection of wet-etch microneedle-treated skin. Bar = 100 xm. 

Fig. 12.3. Epidermal measurements, mitotic figures, and apoptotic keratinocytes in a chronic proliferative dermatitis mutant (Sharplncpdm/Sharplncpdm) mouse. Routine hematoxylin- and eosin-stained paraffin histologic sections can be used to determine proliferation rates based on mitotic index (number of mitotic figures, circled in the figure, in the stratum basale per 1000 cells) or the presence and numbers of apoptotic epidermal keratinocytes (dotted arrows) when present. Epidermal thickness can be measured at high dry magnification (40x) to include the malpigian, living cell, layer (M), the stratum corneum thickness (SC), or the full thickness of the epidermis (M+SC).

Representative hematoxylin- and eosin-stained cross sections from (A) uninjured, (B) vehicle-treated, and (C) paclitaxeI-treated, injured rat carotid arteries. X240. Source. From Ref. 47. 

FIGURE 41.4. Biphasic inflammatory response in the MEVM hematoxylin and eosin stained paraffin sections of mouse ears with (right, 168h post-exposure) and without (left, carrier solvent, alone). Note the edema in the treated ear. The 168h post-SM sample had a very large inflammatory cell infiltration (purple nuclei of thousands of infiltrating cells is apparent). 

Figure 4. Kidney biopsy demonstrates a diffuse cellular infiltrate within the interstitium with inflammatory cells including eosinophils (arrowhead) and lymphocytes. Tubulitis is present (arrow). Hematoxylin and eosin stain (H Ex 47)...

Figure 4. A. Hematoxylin and eosin-stained skin biopsy showing increased spindled fibrocytes between thickened collagen bundles in a patient with NSF (x25). B. A higher magnification showing the spindle cells between broad collagen bundles (xlOO). C. Immunohistochemical stain forCD34-positive dendritic cells between collagen bundles, a characteristic feature of NSF.

Each paraffin-embedded section is collected on microscope slides and first examined under a microscope (lOx) to ensure that it contained sufficient tumor material and to eliminate possible contaminating normal tissues. Tumor and tumor-free areas are identified within 15 pm-thick deparaffinized sections lightly counterstained with hematoxylin and microdissected by gentle scraping with sterile scalpels into 1.5 ml polypropylene vials, using a hematoxylin and eosin-stained step section from the same block [4-7]. 

Figu re 3.9 Photomicrographs of hematoxylin and eosin-stained tissue sections (a, d) and FT-IR microscopic images (b-c, e-f) of consecutive unstained tissue sections obtained from a patient with squamous cell cervical carcinoma. Transition zone from... 

Repeated exposure of sensitized mice to nebulized OVA (OVA mice) without SEB contact induced bronchial inflammation characterized by mainly eosinophils in the BAL fluid and influx of inflammatory cells in bronchial tissue, as illustrated on hematoxylin-and-eosin-stained sections. When SEB was administered onto the nasal mucosa during the development of bronchial allergic inflammation in response to OVA inhalation, bronchial inflammation was clearly aggravated. Compared to nasal application of SEB in OVA mice, bronchial administration of SEB in OVA mice equally aggravated the inflammatory response in the lower airways. Thus, in bronchial tissue of OVA mice, the inflammatory response was aggravated by SEB contact via the nose and the bronchi. 

The PCR-based tissue identity test requires a very small amount of DNA and can be successfully performed using FFPE tissue samples or hematoxylin and eosin-stained tissue fragments removed from glass slides. 

The great majority of fungi are readily identified by hematoxylin and eosin staining alone or in combination with histochemical stains (e.g., periodic acid-Schiff... 

FIGURE 8.16 Urachal cyst (hematoxylin and eosin stain) (A). CDX2 positive nuclei in this urachal remnant (B) from the dome of the bladder confirm the gastrointestinal origin of this cyst. 

Tumors that can be recognized by routine hematoxylin and eosin-stained slides without adjunctive immunohis-tochemistry or molecular analysis are not covered. 

The majority of salivary gland tumors can be diagnosed by routine hematoxylin- and eosin-stained slides. Immu-nohistochemistry may play a role in the diagnosis of some tumors, especially to identify myoepithelial cells... 

FIGURE 14.10 A, Gastric signet-ring cells are relatively inconspicuous within the lamina propria. B, CK20 reveals the numerous neoplastic signet-ring cells, which were not easily seen on hematoxylin and eosin stain. 

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