Hematoxylin stain

Iron hematoxylin Stain or dye to identify cell nuclei or identifiable characteristics of an organism. 

A number of staining procedures have been described. Some stains, such as chlorazol black, require fresh specimens and are not widely used. A variety of stains for fecal smears preserved by Schaudinn or PVA fixative have been described, including various hematoxylin stains. The stain most widely used in the United States is the Wheatley trichrome stain, which is the only permanent stain described in this chapter. The trichrome staining procedure uses reagents with a relatively long shelf life and is easy to perform. Note that there are differences in staining times depending on whether the specimen is fixed in Schaudinn or PVA fixative, as penetration is slower in the latter. 

Figure 2. Photomicrographs (a) and (b) show low and high power views, respectively of a phosphotungstic acid hematoxylin-stained 5 yum thick section of lung from a mouse given a lethal dose of toxin-LR, and then killed when moribund. The arrow in (a) points to a thrombus in a pulmonary artery. The same thrombus is shown at higher magnification in (b). The thrombus consists of irregular clusters of small granules.

FIGURE 7 CFU-F Evaluation of MSCs depends on BM seeding density. An appropriate dilution of the heparinised BM aspiration is needed for accurate enumeration of the primary CFU-F frequency as indicated in this representative experiment where whole heparinized BM was seeded corresponding to the respective measured BM-MNC number per square centimeters of growth area, cultured for 11 days at 37°C/humidified atmosphere/3% 02/5% C02. Nonadherent cells were removed at day 3. CFU-F are visualized by Harris hematoxylin staining. 

Fig. 31-2. Lung of a rhesus monkey that died of inhaled staphylococcal enterotoxin B (SEB) intoxication, (a) Intraalveolar fibrin deposition and hyaline membrane formation can be seen (hematoxylin-eosin stain, original magnification x 50). (b) Intraalveolar deposition of polymerized fibrin can be seen (phosphotungstic acid hematoxylin stain, original magnification X 50).

Ple6nik (1902) was among the first to apply Weigert s chrome-hematoxylin stain to adrenal glands fixed for a prolonged period in potassium dichromate mixtures. He found that the droplets in the adrenal cortex stained intensely after such mordanting, as did those of the interstitial cells in the testis, but that ordinary fat failed to stain. 

Foster, P. Method for improving the shelf-life of hematoxylin staining solutions. U.S. Pat. Appl. Puhl. US 2008139827, 2008 Ghent. Abstr. 2008, 149, 48203. 

Mounting media any available permanent resinous medium for DAB/hematoxylin-stained preparations fluorescence-free aqueous mounting media for fluorescence or confocal laser microscopy (e.g.. Fluorescent Mounting Medium, Dako). 

---