Staining eosin

Hematoxylin/eosin stains of biopsies of back skin taken 24 h post-chemical peeling, a Glycolic acid peel 70%. Note stratum corneum necrosis... 

Fig. i3.2b-d. Hematoxylin/eosin stains of biopsies of c Twenty-five percent TCA induced mid-epidermal back skin taken 24 b post-chemical peeling, b Salicylic wounding/separation. d Thirty percent TCA peel caused acid 30%. Note mild lymphohistiocytic infiltrate, deep epidermal separation... 

FIGURE 21.1 (See color insert following page 336.) Normal tissue from human prostate showing secretory section—hematoxilin and eosin staining showing epithelial cells lining secretory ducts backed by basal and stromal cells. (Courtesy of A. Brollo, Wikimedia Commons, 2005.)... 

Fig. 18.2. Histological appearance of haematoxylin and eosin-stained jejunum of (A) uninfected control, with villi and crypts of normal lengths (B) day 13 p.i.

Other zinc solutions, free of formaldehyde, have been proposed.29 31 All of these simple buffered salt solutions preserve immunoreactivity well and are suitable for DNA, RNA, and proteomics research. Judging by published photomicrographs of hematoxylin and eosin-stained specimens, cytological detail is inferior to that achieved with standard formalin. Nuclei are condensed to the point where many lack chromatin patterns.3132 Such zinc salt solutions may be good for specialized purposes but are best used as special fixatives. To get good structural detail as well, specimens should be split so that a portion can... 

Figure 21.4 (a) Hematoxylin and eosin staining of rat brain coronal section, and... 

Because myoepithelial cells are not always readily identifiable on routine hematoxylin- and eosin-stained sections, many immunohistochemical methods have been used to highlight an intact myoepithelial cells layer. Recent studies have reported CD10 and smooth muscle myosin heavy chain (SMMHC) expression in... 

In another liver foci study using Sprague-Dawley (Crl CD) rats, 1,2-dibromoethane in corn oil given by gavage was used as an initiator. Two dose regimens were used 75 mg/kg 1,2-dibromoethane at 0 and 24 hours or corn oil at 0 hours and 75 mg/kg 1,2-dibromoethane at 24 hours. Partial hepatectomies and phenobarbital in drinking water also were part of the protocol. With this system, at 16 months, 1,2-dibromoethane-exposed rats had increased numbers of foci of hepatic cellular alteration. Rats that received the two doses of 1,2-dibromoethane had increased numbers of nodules on hematoxylin and eosin-stained sections as well as increased number and size of GGT positive foci (Moslen 1984). These results indicate that 1,2-dibromoethane can act as an initiator. 

The presence of necrotic, inflammatory, and/or nontumorigenic tissue should be determined by a pathologist by reviewing the hematoxylin- and eosin-stained 5-pm section prepared in step 1. 

Fig. 11. Megacaryocytes of the spleen (a) animals infected with influenza virus (b) animals infected with influenza virus that were administered MFPC Grinization . Hematoxilin-eosin staining, magnification x 400.

Fig. 13. Liver of A/PR/8/34(HlNl) infected, (a) Intralobular protein-hydropic and ballon degeneration of the liver, (b) Small intralobular infiltrates and marked reaction of hepatic macrophages, (c) Polimorphysm of hepatocytes nuclei, (d) Mononuclear infiltration in the wall of the central vein. Hematoxilin-eosin staining, magnification x 400.

Fig. 16. Acute endocarditis in virus A/PR/8/34(HlNl) infected animal, the third day. Hematoxilin-eosin staining, magnification X 200.

Male Fischer 344 rats were fed diets containing 200 ppm AAF for seven weeks to induce hepatocellular altered foci, and were then fed diets containing either 0 or 12 000 ppm di(2-ethylhexyl) phthalate (purity, 98%) for 24 weeks. In foci that were induced by AAF, di(2-ethylhexyl) phthalate reduced the activity of y-GT, as detected histo-chemically, but did not increase the number, mean volume or volume percentage of foci detected by deficiencies in iron storage, glucose-6-phosphatase, adenosine triphosphatase or fibronectin. Although the numbers of haematoxylin/eosin-stained foci were increased in di(2-ethylhexyl) phthalate-treated rats, the volume percentage was not... 

Figure 21.2 Histochemical analysis of / -galactosidase gene expression in liver. Mice were injected with 1.6ml saline containing various amounts of pCMV-LacZ plasmid DNA. Animals were sacrificed eight hours post injection and liver sections were made using cryostat. Sections (A, B, C and D) were stained with X-gal solution followed by eosin for counter-stain. Sections (E, F, G and H) were stained by a standard hematoxylin/eosin staining method. Sections were made from animals each receiving 0 (A, E), 0.5 (B, F), 2.5 (C, G) and 25 pg (D, H) of pCMV-LacZ. (25x). (see Color Plate 14)...

FIGURE 18.5 Light photomicrographs of microneedle-treated human skin stained for (3-galactosidase expression, (a) En face stereomicroscopy of dry-etch microneedle-treated skin with nuclear fast red counterstaining (b) en face stereomicroscopy of wet-etch microneedle-treated skin (c) hematoxylin and eosin stained 12 xm cryosection of dry-etch microneedle-treated skin. Bar =100 xm (d) hematoxylin and eosin stained 12 xm cryosection of wet-etch microneedle-treated skin. Bar = 100 xm. 

Fig. 12.3. Epidermal measurements, mitotic figures, and apoptotic keratinocytes in a chronic proliferative dermatitis mutant (Sharplncpdm/Sharplncpdm) mouse. Routine hematoxylin- and eosin-stained paraffin histologic sections can be used to determine proliferation rates based on mitotic index (number of mitotic figures, circled in the figure, in the stratum basale per 1000 cells) or the presence and numbers of apoptotic epidermal keratinocytes (dotted arrows) when present. Epidermal thickness can be measured at high dry magnification (40x) to include the malpigian, living cell, layer (M), the stratum corneum thickness (SC), or the full thickness of the epidermis (M+SC).

Fig. 19 Histological preparation (haematoxylin and eosin staining) of square sections, a BASYC in the middle region of the interposition four weeks after implantation in the carotid artery of the rat, b untreated carotid artery of the rat. - endothelial cell layer. BASYC layer...

Representative hematoxylin- and eosin-stained cross sections from (A) uninjured, (B) vehicle-treated, and (C) paclitaxeI-treated, injured rat carotid arteries. X240. Source. From Ref. 47. 

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