Hematoxylin and eosin

For light microscopic examination, liver tissue was fixed in 10 % buffered formalin, embedded in paraffin, sectioned and stained with hematoxylin and eosin. In some cases, preparations were stained with PTAH (phosphotungstic acid-haematoxylin), by the Van Gieson method and the PAS (periodic acid-Schiff)... 

Most mycologists consider it fruitless to directly examine clinical specimens for the yeast form of Sporothrix schenckii. The number of yeast cells in the specimen is typically very limited, and their small size and shape are not distinctive. The yeast form of S. schenckii is not easily seen in sections of tissue stained with hematoxylin and eosin. 

PCNA, proliferating cell nuclear antigen HE, hematoxylin and eosin. 

Figure 10.7 Demonstration of protein dots and tissue on single slide. This specimen was subjected to antigen retrieval prior to immunostaining, and a complete hematoxylin and eosin counterstain. 

Other zinc solutions, free of formaldehyde, have been proposed.29 31 All of these simple buffered salt solutions preserve immunoreactivity well and are suitable for DNA, RNA, and proteomics research. Judging by published photomicrographs of hematoxylin and eosin-stained specimens, cytological detail is inferior to that achieved with standard formalin. Nuclei are condensed to the point where many lack chromatin patterns.3132 Such zinc salt solutions may be good for specialized purposes but are best used as special fixatives. To get good structural detail as well, specimens should be split so that a portion can... 

Figure 21.4 (a) Hematoxylin and eosin staining of rat brain coronal section, and... 

Because myoepithelial cells are not always readily identifiable on routine hematoxylin- and eosin-stained sections, many immunohistochemical methods have been used to highlight an intact myoepithelial cells layer. Recent studies have reported CD10 and smooth muscle myosin heavy chain (SMMHC) expression in... 

Histology. Whole mussels were fixed for 1-2 days in Helly s fluid (10) and stored in 70% ethanol. Tissue was blocked at 2 mm thickness, embedded in paraffin, sectioned at about 7 ym, stained with hematoxylin and eosin, and mounted on glass slides using standard procedures. 

In another liver foci study using Sprague-Dawley (Crl CD) rats, 1,2-dibromoethane in corn oil given by gavage was used as an initiator. Two dose regimens were used 75 mg/kg 1,2-dibromoethane at 0 and 24 hours or corn oil at 0 hours and 75 mg/kg 1,2-dibromoethane at 24 hours. Partial hepatectomies and phenobarbital in drinking water also were part of the protocol. With this system, at 16 months, 1,2-dibromoethane-exposed rats had increased numbers of foci of hepatic cellular alteration. Rats that received the two doses of 1,2-dibromoethane had increased numbers of nodules on hematoxylin and eosin-stained sections as well as increased number and size of GGT positive foci (Moslen 1984). These results indicate that 1,2-dibromoethane can act as an initiator. 

Morphological examination using light microscopy and appropriate staining (e.g., hematoxylin and eosin, H E) [69],... 

Figure 21. Morphology of rat aortic smooth muscle cells on day 1 (A, B) and 3 (C, D) after seeding on pristine unmodified PE foils (A, C) or foils irradiated with 3xl0 ions C+ cm-2 (B) or 3xl0 " ions O cm (D) energy 150 keV. Stained with hematoxylin and eosin, microscope Opton Axioplan, Leica, Germany. Bar=100 pm.

Figure 22. Human embryonic kidney cells (A), rat vascular smooth muscle cells (B, C) and human osteoblast-like MG 63 cells (D) in cultures on micropattemed surfaces. A, B PTFE irradiated with UV light produced by a Xe2 -excimer lamp for 30 min in an ammonia atmosphere through a mask with holes 100 pm in diameter and center-to-center distance 300 pm C PE irradiated with Ar ions (energy 150 keV, ion dose lO ions/cm ) through a mask with holes 100 pm in diameter and center-to-center distance 200 pm fullerenes Qo deposited through a mask with rectangular holes with an average size of 128 3 pm per 98 8 pm on glass coverslips. Day 7 after seeding. A native cells in an inverted phase-contrast microscope B, C cells stained with hematoxylin and eosin, Olympus microscope IX 50 D cells stained with fluorescence-based LIVE/DEAD viability/cytotoxicity kit (Invitrogen), Olympus microscope IX 50. Bars 300 pm (A), 200 pm (B, D), Imm (C) [10,11].

The presence of necrotic, inflammatory, and/or nontumorigenic tissue should be determined by a pathologist by reviewing the hematoxylin- and eosin-stained 5-pm section prepared in step 1. 

Fix the inserts with 4% paraformaldehyde, and then stain with hematoxylin and eosin (H8cE) for 2 min or Diff-Quick staining solutions, according to the manufacturer s instructions. 

FIGURE 2.2 Inhibitory effects of CS on TPA-induced edema and expression of VCAM-1 and COX-2 in mouse skin. Mice were treated topically with TPA (200 pL of 10 nmol of TPA dissolved in acetone) in the presence or absence of CS (1 or 2mg). Control animals were treated with acetone alone. (A) Skin section was stained with hematoxylin and eosin (original magnification, xlOO bar, 100 pm) (B) thickness of the skin (the thickness of the ear of each mouse was determined by averaging the values measured at five independent... 

FIGURE 18.5 Light photomicrographs of microneedle-treated human skin stained for (3-galactosidase expression, (a) En face stereomicroscopy of dry-etch microneedle-treated skin with nuclear fast red counterstaining (b) en face stereomicroscopy of wet-etch microneedle-treated skin (c) hematoxylin and eosin stained 12 xm cryosection of dry-etch microneedle-treated skin. Bar =100 xm (d) hematoxylin and eosin stained 12 xm cryosection of wet-etch microneedle-treated skin. Bar = 100 xm. 

One of the most commonly used staining procedures involves hematoxylin and eosin, but it was found that the dyes interfere with either the proteins or the MALDI process. As a consequence, the quality of the mass spectra is significantly compromised [54], When five other staining protocols (Terry s Polychrome, Toluidine Blue, Nuclear Fast Red, Cresyl Violet, and Methylene Blue) were compared to a control section (unstained tissue rinsed in 70% and 100% ethanol), Cresyl Violet and Methylene Blue had the highest degrees... 

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