Glucose oxidase -based biosensors

Determination of methyl mercury in fish tissue using electrochemical glucose oxidase biosensors based on invertase inhibition... 

Glucose Oxidase Biosensor Based on Colloidal Gold-Carbon Nanotubes... 

Zhu, M. Jiang, Z. Jing, W. Fabrication of polypyrrole-glucose oxidase biosensor based on multilayered interdigitated ultramicroelectrode array with containing trenches. Sens. Actuators B Chem. 2005, 2, 382-389. 

Determination of methyl mercury using biphasic system (aqueous solution/ solvent) in the presence of fish matrix. The method is based on enzyme inhibition combined with glucose oxidase biosensor... 

The principle of combination of electrochemical glucose oxidase biosensor with the clean-up method for direct extraction and determination of methyl mercury has been successfully demonstrated. The extraction of methyl mercury from the organic solvent has been based on invertase enzyme inhibition. The combination of very low concentration of invertase enzyme and 10 min of incubation time allows the detection of methyl mercury at 5 ppb level. Our method permits the detection of this inhibitor below the legal limit given by the European Union with good recoveries when fish samples were measured. 

Several biosensors are commercially available. One of the most useful is the glucose sensor. The standard sensor determines glucose concentration based on the glucose oxidase enzyme. The chemical reaction for oxidation of glucose is ... 

Later on, such S-layer-based sensing layers were also used in the development of optical biosensors (optodes), where the electrochemical transduction principle was replaced by an optical one [97] (Fig. 10c). In this approach an oxygen-sensitive fluorescent dye (ruthenium(II) complex) was immobilized on the S-layer in close proximity to the glucose oxidase-sensing layer [97]. The fluorescence of the Ru(II) complex is dynamically quenched by molecular oxygen. Thus, a decrease in the local oxygen pressure as a result of... 

Tan, X.C.,Tian, Y.X., Cai, P.X. andZou, X.Y. (2005) Glucose biosensor based on glucose oxidase immobilized in sol—gel chitosan/silica hybrid composite film on Prussian blue modified glass carbon electrode. Analytical and Bioanalytical Chemistry, 381, 500-507. 

Sensors for glucose are most important and have received most attention. The preferred format still is based on the use of glucose oxidase (GOx). Other glucose biosensors using immobilized glucose oxidase132 134 or a pH... 

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X.J. Wu and M.M.F. Choi, An optical glucose biosensor based on entrapped-glucose oxidase in silicate xerogel hybridised with hydroxyethyl carboxymethyl cellulose. Anal. Chim. Acta 514, 219-226 (2004). 

P.C. Pandey, S. Upadhyay, N.K. Shukla, and S. Sharma, Studies on the electrochemical performance of glucose biosensor based on ferrocene encapsulated ORMOSIL and glucose oxidase modified graphite paste electrode. Biosens. Bioelectron. 18,1257—1268 (2003). 

S.Q. Liu and H.X. Ju, Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens. Bioelectron. 19, 177-183 (2003). 

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