Gene identification

Databases Comparison of DNA and protein sequences obtained from unknown gene with known sequences in databases can facilitate gene identification. 

Erdmann, J., Shimron-Abarbanell, D., Rietschel, M. etal. (1996). Systematic screening for mutations in the human serotonin-2A (5-HT2a) receptor gene identification of two naturally occurring receptor variants and association analysis in schizophrenia. Hum. Genet., 97, 614-19. 

Bai, C., and Elledge, S. J. (1997). Gene identification using the yeast two-hybrid system. Methods Enzymol. 283, 141-156. 

Botka, C. W., et al. Human proton/ oligopeptide transporter (POT) genes identification of putative human genes using bioinformatics. AAPS PharmSci. 2000, 2, E16. 

S, Papiha SS, Dai R, et al. The human peroxisome proliferator-activated receptor alpha gene identification and functional characterization of two natural allelic variants. Pharmacogenetics 2000 10 321-333. 

SEQUENCE-BASED APPROACHES TO ALKALOID BIOSYNTHESIS GENE IDENTIFICATION... 

This chapter is an historical perspective with selected examples from our own laboratory, first at the University of Munich, and now at the Leibniz Institute of Plant Biochemistry in Halle, that reflect the changes in how we define and exploit sequence-based approaches to alkaloid biosynthesis gene identification. ... 

Sequence-Based Approaches to Alkaloid Biosynthesis Gene Identification... 163 Toni M. Kutchan... 

O Malley KL, Anhalt MJ, Martin BM, Kelsoe JR, Winfield SL, et al. 1987. Isolation and characterization of the human tyrosine hydroxylase gene identification of 5 alternative splice sites responsible for multiple mRNAs. Biochemistry 26 6910-6914. 

Rao Y, Hoffmann E, Zia M, Bodin L, Zeman M, Sellers EM, Tyndale RE (2000) Duplications and defects in the CYP2A6 gene identification, genotyping, and in vivo effects on smoking. Mol Pharmacol 58 747-755... 

Dn Y., Spence S.E., Jenkins N.A. and Copeland N.G. (2005) Cooperating cancer-gene identification throngh oncogenic-retrovirns-indnced insertional mutagenesis. Blood 106, 2498-2505. 

Tsou AP, Wu KM, Tsen TY, Chi CW, Chiu JH, Lui WY et al (1998). Parallel hybridization analysis of multiple protein kinase genes identification of gene expression patterns characteristic of human hepatocellular carcinoma. Genomics 50 331 340. 

Suzuki, T., Minehata, K., Akagi, K., Jenkins, N.A. and Copeland, N.G. (2006) Tumor suppressor gene identification using retroviral insertional mutagenesis in Blm-defident mice. The EMBO Journal, 25, 3422-3431. 

Although current methods for the detection of gene-environment interaction in behavioral genetics are largely indirect, the most pertinent consequence of gene identification for behavioral traits may be that it will provide the tools required to systematically elucidate the effects of gene-environment interaction. 

Reliable criteria to identify the (individual) patients suitable for high-LET radiation therapy need to be developed. At present, the available criteria are derived (mainly) from the clinical fast neutron experience. It can be expected that novel approaches based on modern techniques involving molecular biology or gene identification may provide appropriate and still missing information. They may also provide information on the susceptibility or risk for secondary radio-induced cancer. 

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