Gene identification sequence analysis techniques

There are two approaches in the development of sequence analysis techniques for gene identification (Fickett, 1996 Brent and Guigd, 2004) ab initio and de novo methods. The ab initio gene prediction is based only on the genome sequences using the statistical... 

ML techniques are well situated for the analysis of molecular sequence data. These methods have been applied successfully to a variety problem, ranging from gene identification to protein structure prediction and sequence classihcation (24,25). These techniques have become an important topic for... 

Allelic polymorphism in a specific gene can produce nearly identical proteins with primary sequences that differ by one or more amino acids. Primary sequence isoforms can be readily separated by CIEF on the basis of their pi if the amino acid substitution changes the surface charge of the protein. Similarly, enzymatic or nonenzymatic posttranslational chemical modification of ionizable protein functional groups can produce chemically distinct isoforms of the same protein with detectably different surface charge and pi. Analysis of hemoglobin by CIEF is an excellent example of how this technique can be applied for the identification and quantification of a family of related proteins that represent a concise subset of the human proteome. Members of this family include many primary... 

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