RRNA genes, bacteria identification

The discovery that rRNA plays an active role in all the functions of the ribosome, as well as the observation that there is a core in the rRNA molecules that has been conserved in all organisms, has given strong support to Woese s proposal [75] that the most primitive ribosomes were composed solely of RNA. On the genetic level, the identification of internal transcribed spacers (ITS) [47] or intervening sequences (IV S) [48] in the rRNA genes of bacteria and eucarya has provided us with a... 

Widjojoatmodjo MN, Fluit AC, Verhoef J (1995) Molecular identification of bacteria by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene. J Clin Microbiol 33 2601-2606... 

Mellmann, A., Qoud,)., Maier, T Keckevoet, U., et al. (2008) Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria. /. Clin. Microbiol, 46,1946-1954. 

Han SK, Hong Y, Kwak HL, Kim ES, Kim MJ, Shrivastav A, Oh MH, Kim HY Identification of lactic acid bacteria in pork meat and pork meat products using SDS-PAGE, 16S rRNA gene sequencing and MALDl-TOF mass spectrometry. J Food Safety. 2014 34(3) 224-32. 

Trcek J, Barja F (2015) Updates on quick identification of acetic acid bacteria with a focus on the 16S-23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry. Int J Food Microbiol 196 137-144 Trcek J, Raspor P, Teuber M (2000) Molecular identification of Acetobacter isolates fiom submerged vinegar production, sequence analysis of plasmid pJK2-l and application in development of a cloning vector. Appl Microbiol Biotechnol 35 1899-1901 Trcek J, Toyama H, Czuba J, Misiewicz A, Matsushita K (2006) Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria. Appl Microbiol Biotechnol 70(3) 366-373... 

Table 2.1. Filarial nematodes infected with intracellular bacteria. The method of detection is shown. Neither electron microscopy nor the immuno-histochemical staining techniques used are to be regarded as Wolbachia specific (see note). Positive identification of intracellular bacteria as Wolbachia is shown only where PCR amplified products of rRNA or ftsZ genes have been sequenced. ...

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