Fluorescence microspectroscopy

Kim, H.-B., Hayashi, M., Nakatani, K., Kitamura, N., Sasaki, K., Hotta, J.-I., and Masuhara, H., In situ measurements of ion exchange processes in single polymer particles laser trapping microspectroscopy and confocal fluorescence microspectroscopy, Anal. Chem., 68, 409, 1996. 

West, S.S. Fluorescence microspectroscopy of mouse leukocytes supravitally stained with acridine orange. Acta Histochemica, Suppl. 6 135-153, 1965. 

The Brownian motion of microdroplets is vigorous in solution. The volume of a micrometre-sized droplet is 10 - 10 dm. Therefore, a manipulation technique is indispensable for single microdroplet measurements. For the microanalysis of a single microdroplet, size of the probe should be smaller than that of the microdroplet. A light beam and a microelectrode are frequently used as a probe, and the analyses of small domains are performed by absorption/fluorescence microspectroscopy [24—29] and mi-croelectrochemical methods [17,30-32], In this section, single microdroplet techniques for the kinetic analysis of physical and chemical processes across a microdroplet/solution interface are described. 

FIGURE 9.1. Block diagram of laser and microcapillary manipulation, absorption/fluorescence microspectroscopy and microelectrochemistry system. 

Recently, the micro-two-phase sheath flow method (Figure 10.3) has been invented. Fast interfaeial reactions, which proceeded in less than 1 millisecond, were measured using this method, coupled with fluorescence microspectroscopy. An inner organic micro-flow was generated in the aqueous phase flowing from the tip of a capillary (i.d.. 

Size-dependent fluorescence spectra of individual perylene nanocrystals studied by far-field fluorescence microspectroscopy coupled with atomic force microscope observation. Polish J. Chem., 84, 687-699. 

I.C. Baianu, D. Costescu, T. You, P.R. Lozano, N.E. Hofmann and S.S. Korban, Near Infrared Microspectroscopy, Fluorescence Microspectroscopy, Infrared Chemical Imaging and High-Resolution Nuclear Magnetic Resonance Analysis of Soybean Seeds, Somatic Embryos and Single Cells , Los Alamos National Laboratory, Preprint Archive, Quantitative Biology, Avail. URL http //xxx.lanl.gov/pdf/ q-bio.TO/0407006... 

In a related study [47], the same group used steady-state fluorescence spectroscopy coupled to epifluorescence microscopy (fluorescence microspectroscopy) for studying the diffusion of the lower-component during the heating of a binary... 

Action Spectroscopy Absorption and Fluorescence Microspectroscopy Biochemical and Spectroscopic Study of Extracted Visual Pigments Molecular Biology Investigations... 

Fisz JJ (2009) Another treatment of fluorescence polarization microspectroscopy and imaging. J Phys Chem A 113 3505-16... 

In this chapter we explore several aspects of interferometric nonlinear microscopy. Our discussion is limited to methods that employ narrowband laser excitation i.e., interferences in the spectral domain are beyond the scope of this chapter. Phase-controlled spectral interferometry has been used extensively in broadband CARS microspectroscopy (Cui et al. 2006 Dudovich et al. 2002 Kee et al. 2006 Lim et al. 2005 Marks and Boppart 2004 Oron et al. 2003 Vacano et al. 2006), in addition to several applications in SHG (Tang et al. 2006) and two-photon excited fluorescence microscopy (Ando et al. 2002 Chuntonov et al. 2008 Dudovich et al. 2001 Tang et al. 2006). Here, we focus on interferences in the temporal and spatial domains for the purpose of generating new contrast mechanisms in the nonlinear imaging microscope. Special emphasis is given to the CARS technique, because it is sensitive to the phase response of the sample caused by the presence of spectroscopic resonances. 

Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and nonlinear Raman microspectroscopy. The text explores the promise of beam shaping and the use of a broadband laser pulse generated through continuum generation and an optical pulse shaper. 

A series of advances over the past decade have made CRS microscopy a highly sensitive tool for label-free imaging and vibrational microspectroscopy that is capable of real-time, non-perturbative studies of complex biological samples based on molecular Raman spectroscopy. In particular, biomedical applications where fluorescent labeling of small molecules represents a severe pertur-... 

G. J. Puppels, Confocal Raman Microspectroscopy in Fluorescent and Luminecent Probes for Biological Activity (W. T. Mason, ed.), Life Sci. Resources Ltd. Cambridge, UK (1999). 

Peters, R., and Scholz, M. (1991). Fluorescence photobleaching techniques. In New Techniques of Optical Microscopy and Microspectroscopy (R. J. Cherry, ed.), pp. 199-228. Macmillan, London. 

Large coffin-shaped zeolite H-ZSM-5 crystals have been chosen as suitable model systems to study the structural motifs and related intergrowth structure, which are also present in smaller powdered zeolite materials used in industrial catalysis. The advantage of large model crystals, which can be prepared with adjustable Si/Al ratio and size, is that the catalytic properties of each growth unit of a zeolite can be studied separately by means of optical microscopy methods, such as UV-vis microspectroscopy and confocal fluorescence microscopy [114, 122]. As both microspectroscopy methods obey the optical Abbe diffraction limit, features can be studied in the size of approximately X/2. The resolution d of an optical microscope is given by... 

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