Third harmonic generation microscopy

Millard, A. C., Wiseman, P W., Fittinghoff, D. N., et al. 1999. Third harmonic generation microscopy by use of a compact femtosecond fiber laser source. Appl. Opt. 38 7393. 

Squier, J. A., Muller, M., Brakenhoff, G. J., and Wilson, K. R. 1998. Third harmonic generation microscopy. Review of dynamic imaging with THG in living organisms was first demonstrated. The point scanning source was used for THG imaging. Excitation at 1.2 (xm, 250 kHz. Interface orientation dependency in respect to the laser beam was shown in glass beats. Opt. Exp. 3 315-24. 

Yelin, D., and Silberberg, Y. 1999. Laser scanning third-harmonic-generation microscopy in biology. Opt. Exp. 5 169-75. 

Debarre, D., Pena, A. M., Supatto, W., Boulesteix, T., Simpler, M., Sauviat, M. R, Martin, J. L., Schanne-Klein, M. C., and Beaurepaire, E. 2007. Second- and third-harmonic generation microscopies for the structural imaging of intact tissues. Med. Sci. 22 845-50. 

Debarre, D., Supatto, W., and Beaurepaire, E. 2005. Structure sensitivity in third-harmonic generation microscopy. Opt. Lett 30 2134-36. 

Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and nonlinear Raman microspectroscopy. The text explores the promise of beam shaping and the use of a broadband laser pulse generated through continuum generation and an optical pulse shaper. 

Similar approaches were adopted by Ganikhanov (Chapter 5), who developed a state-of-the-art laser system, benefiting simultaneous third-harmonic and nonlinear Raman microscopy, and Yakovlev et al. (Chapter 6), who applied third-harmonic generation microscopy and nonlinear Raman microspectroscopy for biochemical analysis in microfluidic devices. 

Debarre D, Supatto W. Pena AM, Fabre A, Tordjmann T, Combettes L, Schanne-Klein MC, Beaurepaire E (2006) Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy. Nat Methods 3 47-53... 

Schaller, R. D., Johnson, J. C Wilson, K R., Lee, L. F., Haber, L. H. and Saykally, R. J. (2002) Nonlinear chemical imaging nanomicroscopy from second and third harmonic generation to multiplex (broad-bandwidth) sum frequency generation near-freld scanning optical microscopy. 

Barad, Y., Eisenberg, H., Horowitz, M., and Silberberg, Y. 1997. Nonlinear scanning laser microscopy by third harmonic generation. Appl. Phys. Lett. 70 922-24. 

Greenhalgh, C., Cisek, R., Prent, N., Major, A., Aus der Au, J., Squier, J., and Barzda, V. 2005. Time and structural image analysis of microscopic volumes, simultaneously recorded with second harmonic generation, third harmonic generation, and multiphoton excitation fluorescence microscopy. Proc. SPIE 5969 59692F1-F8. 

Muller, M., Squier, J., Wilson, K. R., and Brakenhoff, G. J. 1998a. 3D microscopy of transparent objects using third-harmonic generation. J. Microsc. 191 266-74. 

Naumov, A. N., Sidorov-Biryukov, D. A., Fedotov, A. B., and Zheltikov, A. M. 2001. Third-harmonic generation in focused beams as a method of 3D microscopy of a laser-produced plasma. Opt. Spectrosc. 90 778-83. 

By taking advantage of the deep penetration depth offered by CARS microscopy with near-infrared laser pulses, CARS tissue imaging has been demonstrated to complement other label-free optical techniques, such as auto-fluorescence that is limited to a few chemical species and optical tomography, second harmonic generation (SHG), and third harmonic generation (THG)... 

TEM TERS THG THz TOF TXM Transmission Electron Microscope Tip-enhanced Ramen Spectroscopy Third Harmonic Generation Terahertz Time of Flight Full-field X-ray Microscopy... 

R.D. Schaffer, P.T. Snee, J.C. Johnson, L.F. Lee, K.R. Wilson, L.H. Haber, R.J. SaykaUy, T.Q. Nguyen, and B.J. Schwartz, Nanoscopic interchain aggregate domain formation in conjugated polymer films studied by third harmonic generation near-field scanning optical microscopy. J. Chem. Phys., 117, 6688 (2002). 

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