Stain, supravital

West, S.S. Fluorescence microspectroscopy of mouse leukocytes supravitally stained with acridine orange. Acta Histochemica, Suppl. 6 135-153, 1965. 

CSGMT (1992). Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining the summary report of the 5th collaborative study by CSGMT/JEMS.MMS. The Collaborative Study Group for the Micronucleus Test. Mutat Res 278, 83-98. 

The principle of the test is that, in the presence of complement, a reaction between HLA antigens on lymphocytes and corresponding antibodies in serum will cause activation of complement and consequent lysis of the lymphocytes. This lysis can be detected by the addition of a supravital stain such as eosin or trypan blue. Given an antiserum of known HLA specificity (e.g., HLA-A2) and lymphocytes of unknown specificity, the incorporation of stain into the test lymphocytes following incubation with complement (usually obtained from rabbits) implies that the lymphocytes possess the antigen specificity corresponding to that of the antiserum. By repeating this technique with antisera of different specificities, the HLA phenotype of the test lymphocytes can be ascertained. 

This is a supravital staining method (Busch and Smetana, 1970) used to identify nucleoli during isolation procedures such as those described in Section VII. 

Polychromatophilic erythrocyte (Diffusely basophilic erythrocyte) Absent 0 Mixed acidophilic-basophilic, supravital stains show granulo-filamentous reticulum 1-5... 

Augulis, V Sigg, E. B. Supravital staining and fixation of brain and spinal cord by intravascular perfusion. Stain Technol. 1971, 46, 183-190. 

Korsakova, N. V. Sergeeva, V. E. Cytochemical analysis of cellular structures in eye lens of laboratory animals including vital-supravital staining by methylene blue. Russ. RU 2319132, 2008 Chem. Abstr. 2008, 148, 302857. 

Tanaka, Y. Deposition of Janus Green B and pinocyanol in mitochondria of supravitally stained KB cells. Exp. Cell Res. 1968, 52, 338-348. 

Buehring, G. C. Jensen, H. M. Lack of toxicity of methylene blue chloride to supravitally stained human mammary tissues. Cancer Res. 1983, 43, 6039-6044. 

Traganos, F. Darzynkiewicz, Z. Lysosomal proton pump activity Supravital cell staining with acridine orange differentiates leukocyte subpopulations. Methods Cell Biol. 1994,41,185-194. 

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