Microspectroscopy, absorption/fluorescence

The Brownian motion of microdroplets is vigorous in solution. The volume of a micrometre-sized droplet is 10 - 10 dm. Therefore, a manipulation technique is indispensable for single microdroplet measurements. For the microanalysis of a single microdroplet, size of the probe should be smaller than that of the microdroplet. A light beam and a microelectrode are frequently used as a probe, and the analyses of small domains are performed by absorption/fluorescence microspectroscopy [24—29] and mi-croelectrochemical methods [17,30-32], In this section, single microdroplet techniques for the kinetic analysis of physical and chemical processes across a microdroplet/solution interface are described. 

FIGURE 9.1. Block diagram of laser and microcapillary manipulation, absorption/fluorescence microspectroscopy and microelectrochemistry system. 

Action Spectroscopy Absorption and Fluorescence Microspectroscopy Biochemical and Spectroscopic Study of Extracted Visual Pigments Molecular Biology Investigations... 

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