Fluorescence characterization

In studies of molecular dynamics, lasers of very short pulse lengths allow investigation by laser-induced fluorescence of chemical processes that occur in a picosecond time frame. This time period is much less than the lifetimes of any transient species that could last long enough to yield a measurable vibrational spectrum. Such measurements go beyond simple detection and characterization of transient species. They yield details never before available of the time behavior of species in fast reactions, such as temporal and spatial redistribution of initially localized energy in excited molecules. Laser-induced fluorescence characterizes the molecular species that have formed, their internal energy distributions, and their lifetimes. 

Fluorescence Characterization of Ablated Polymeric Materials. In order to produce sharply etched patterns, the film was ablated with a photo-lithographically prepared mesh mask in the contact mode. The ablation was conducted with two laser shots with the laser fluence of 0.2 J/cm2. The decay curves of the ablated film was measured by a... 

A. Orstan, M. F. Lulka, B. Eide, P. H. Petra, and J. B. A. Ross, The steroid-binding site of human and rabbit sex steroid-binding protein of plasma Fluorescence characterization with... 

E. Friere, T. Markello, C. Rigell, and P. W. Holloway, Calorimetric and fluorescence characterization of interactions between cytochrome bs and phosphatidylcholine bilayers, Biochemistry 22, 1675-1680 (1983). 

The detailed fluorescence characterization of pteridine analogues of adenosine nucleosides has been reported <2001ABI231>. The 4-aminopteridin-7-one 8-nucleosides 215 and 216 had absorption maxima at 310 and 330 nm, respectively, and both emit at 430 nm. The fluorescence was monoexponential with a lifetime of 3.8 and 4.3 ns. 

Mobed, J. J., S. L. Hemmingsen, J. L. Autry, and L. B. McGowan. 1996. Fluorescence characterization of IHSS humic substances total luminescence spectra with absorbance correction. Environmental Science Technology 30 3061—3065. 

Plaza, C., Brunetti, G., Senesi, N., and Polo, A. (2006c). Fluorescence characterization of metal ion-humic acid interactions in soils amended with composted municipal solid wastes. Anal. Bioanal. Chem. 386, 2133-2140. 

Janiak, F., Dell, V. A., Abrahamson, J. K., Watson, B. S., Miller, D. L., and Johnson, A. E. (1990). Fluorescence characterization of the interaction of various transfer RNA species with elongation factor Tu.GTP Evidence for a new functional role for elongation factor Tu in protein biosynthesis. Biochemistry 29, 4268—4277. 

Moreover, fluorescence characterization of these systems indicated, as previously hypothesized, that ruthenium(II) cationic complexes are able to activate energy transfer processes towards the anionic porphyrins [50]. 

Hawkins ME, Pfleiderer W, Jungmann O, and Balisa FM (2001) Synthesis and fluorescence characterization of pteridine adenosine nucleoside analogs for DNA incorporation. Analytical Biochemistry 298 231-240. 

Myers M, Freire E. Calorimetric and fluorescence characterization of interactions between enkephalins and liposomal and synaptic plasma membranes containing gangliosides. Biochemistry 1995 24 4076 082. 

Time-Resolved Laser-Induced Fluorescence Characterization of Uranium Complexes and Processes in ScF CO2... 

Advanced glycation end products attached to proteins (AGE proteins), which can be detected by their characteristic fluorescence, have been found to increase at an accelerated rate in diabetes and with increasing age (Monnier et al., 1984, 1986). However, the chemical and fluorescent characterization of these compounds remains imprecise or incomplete, and many products with similar fluorescent characteristics can be generated by ascorbic acid and unsaturated lipid, in vitro at least (Hunt and Wolff, 1990, 1992). 

R., Vallabhan, C.P.G., Nampoori, V.P.N., and Radhakrishnan, P. (2008) Fluorescence characterization and gain studies on a dye-doped graded index polymer optical-fiber preform. Opt. Laser Tech.,... 

K. Tiefenbacher and H. Woidich, Determination of the Pattern of Polycyclic Aromatic Hydrocarixms (PAH) in Food and Environmental Samples. Cleanup, TLC, in Situ-Fluorescence Characterization, Proc. 3rd Int. Symp. Instrumental High Performance Thin-Layer Chromatography, Wttrzburg, West Germany, 1985, pp. 357-366. 

Kim YS, Sook C, Sung P. UV and fluorescence characterization of styrene and methyl methacrylate polymerization. J Appl Polym Sci 1995 57 363-370. 

Martin G, Ross JA, Minteer SD, Jameson DM, Cooney MJ. Fluorescence characterization of chemical microenvironments in hydrophohically modified chitosan. Carbohydr Polym 2009 77 695-702. 

Kyle D J and Zalik S (1982) Photosystem II activity, plastoquinone A levels, and fluorescence characterization of a virescens mutant of barley. Plant Physiol. 70, 1026-1031. 

Mobed, J.J., Henuningsen, S.L., Autry, J.L., and McGown, L.B. (1996). Fluorescence characterization of IHSS humic substances Total luminescence spectra with absorbance correction. Environ. ScL Technol, 30, 3061-3065. 

Other samples even showed a small increase, resulting in post-irradiation waters having an increased contribution of protein-like relative to humic-like components in comparison to initial waters (Cory et al., 2007). Whole water samples in freshwater systems also typically show a decrease in FI with irradiation. The variation in FI has been shown to be related to relative amounts of SQl and SQ2 in a sample and thus the decrease in H with irradiation time can be attributed to the greater loss of SQ2 in comparison to SQl in Figure 3.10 (Cory et al., 2007). Fluorescence characterization of DOM can help understand how photochemical processes influence DOM quantity and quality, and utihzing spectrophotometric techniques in comparison with other DOM characterization techniques shows promise for understanding how photochemical processes remove and modify DOM in aquatic systems (Spencer et al, 2009a Stubbins et al, 2010). 

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